Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all ALK-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ALK-1
Asp23-Pro119
Accession # Q61288

Specificity

Detects mouse ALK-1 in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross-reactivity with recombinant human ALK‑1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse ALK‑1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse ALK‑1 Fc Chimera (Catalog # 770-MA)

Mouse ALK-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 0.2-0.8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Mouse ALK‑1 Biotinylated Antibody (Catalog # BAF770)
  • Standard: Recombinant Mouse ALK-1 Fc Chimera Protein, CF (Catalog # 770-MA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 3 reviews rated 5 using AF770 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ALK-1

Transforming growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. Evidence suggests that TGF-beta 1, TGF-beta 3 and an unknown ligand present in serum can activate chimeric ALK-1. ALK-1 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine-and serine-rich domain (the GS domain) preceding the kinase domain. ALK-1 is expressed highly in endothelial cells and other highly vascularized tissues. The expression patterns of ALK-1 parallels that of endoglin. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting a critical role for ALK-1 in the control of blood vessel development or repair. Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

References

  1. ten Dijke, P. et al. (1993) Oncogene 8:2879.
  2. ten Dijke, P. et al. (1994) Science 264:101.
  3. Lux, A. et al. (1999) J. Biol. Chem. 274:9984.

Long Name

Activin Receptor-like Kinase 1

Alternate Names

ACVRL1, ALK1

Entrez Gene IDs

94 (Human); 11482 (Mouse)

Gene Symbol

ACVRL1

UniProt

Q61288

Additional ALK-1 Products

Product Documents for Mouse ALK‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse ALK‑1 Antibody

For research use only

Citations for Mouse ALK‑1 Antibody

Customer Reviews for Mouse ALK‑1 Antibody (3)

5 out of 5
3 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
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1 Stars
0%

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Showing  1 - 3 of 3 reviews Showing All
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  • Mouse ALK-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Murine neuroblastoma cells
    Species: Mouse
    Verified Customer | Posted 07/21/2021
    Mouse ALK‑1 Antibody AF770
  • Mouse ALK-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Serum
    Species: Mouse
    Verified Customer | Posted 05/03/2021
    Mouse ALK‑1 Antibody AF770
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22421041
    Species: Mouse
    Verified Customer | Posted 01/09/2015

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