Mouse ALK-1 Antibody

Catalog # Availability Size / Price Qty
AF770
AF770-SP
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (2)

Mouse ALK-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse ALK-1 in ELISAs and Western blots. In sandwich immunoassays, less than 5% cross-reactivity with recombinant human ALK‑1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ALK-1
Asp23-Pro119
Accession # Q61288
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse ALK‑1 Fc Chimera (Catalog # 770-MA)

Mouse ALK-1 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Mouse ALK‑1 Biotinylated Antibody (Catalog # BAF770)

Standard: Recombinant Mouse ALK-1 Fc Chimera Protein, CF (Catalog # 770-MA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK-1

Transforming growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. Evidence suggests that TGF-beta 1, TGF-beta 3 and an unknown ligand present in serum can activate chimeric ALK-1. ALK-1 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine-and serine-rich domain (the GS domain) preceding the kinase domain. ALK-1 is expressed highly in endothelial cells and other highly vascularized tissues. The expression patterns of ALK-1 parallels that of endoglin. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting a critical role for ALK-1 in the control of blood vessel development or repair. Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

References
  1. ten Dijke, P. et al. (1993) Oncogene 8:2879.
  2. ten Dijke, P. et al. (1994) Science 264:101.
  3. Lux, A. et al. (1999) J. Biol. Chem. 274:9984.
Long Name
Activin Receptor-like Kinase 1
Entrez Gene IDs
94 (Human); 11482 (Mouse)
Alternate Names
activin A receptor type II-like 1; activin A receptor, type II-like kinase 1; Activin receptor-like kinase 1; ACVRL1; ACVRLK1ORW2; ALK1; ALK-1; ALK1TGF-B superfamily receptor type I; EC 2.7.11; HHT; HHT2EC 2.7.11.30; serine/threonine-protein kinase receptor R3; SKR3; TSR-I

Product Datasheets

Citations for Mouse ALK-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. PI3 kinase inhibition improves vascular malformations in mouse models of hereditary haemorrhagic telangiectasia
    Nat Commun, 2016;7(0):13650.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. BMP9/ALK1 inhibits neovascularization in mouse models of age-related macular degeneration
    Authors: K Ntumba, N Akla, SP Oh, A Eichmann, B Larrivée
    Oncotarget, 2016;7(35):55957-55969.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  3. Endothelial beta4 integrin is predominantly expressed in arterioles, where it promotes vascular remodeling in the hypoxic brain.
    Authors: Welser-Alves J, Boroujerdi A, Tigges U, Wrabetz L, Feltri M, Milner R
    Arterioscler Thromb Vasc Biol, 2013;33(5):943-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. ALK1-Fc inhibits multiple mediators of angiogenesis and suppresses tumor growth.
    Authors: Mitchell D, Pobre EG, Mulivor AW, Grinberg AV, Castonguay R, Monnell TE, Solban N, Ucran JA, Pearsall RS, Underwood KW, Seehra J, Kumar R
    Mol. Cancer Ther., 2010;9(2):379-88.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Secondary Antibodies

Reviews for Mouse ALK-1 Antibody

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Mouse ALK-1 Antibody
By Anonymous on 07/21/2021
Application: WB Sample Tested: Murine neuroblastoma cells Species: Mouse

Mouse ALK-1 Antibody
By Anonymous on 05/03/2021
Application: WB Sample Tested: Serum Species: Mouse