Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all AMICA/JAML Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse AMICA/JAML
Leu23-Leu281
Accession # Q80UL9

Specificity

Detects mouse AMICA/JAML in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human AMICA is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse AMICA/JAML Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse AMICA/JAML Fc Chimera (Catalog # 3106-AM)

Reviewed Applications

Read 1 review rated 5 using AF3106 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: AMICA/JAML

AMICA, also known as JAML, is a 65 kDa, type I transmembrane glycoprotein that belongs to the junctional adhesion molecule (JAM) subset of the immunoglobulin superfamily (1). JAM family proteins contribute to intercellular connections within epithelial and endothelial cell layers and mediate their interactions with various hemopoietic cells (1). The mouse AMICA cDNA encodes a 379 amino acid (aa) precursor that includes a 20 aa signal sequence, a 261 aa extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 77 aa cytoplasmic domain (2). In contrast to other JAM family proteins, AMICA does not contain a cytoplasmic PDZ-binding motif (3). Within the ECD, mouse AMICA shares 58% and 77% aa sequence identity with human and rat AMICA, respectively. It shares 17%‑23% aa sequence identity with the ECDs of mouse JAM-A, -B, -C, and JAM4. AMICA is expressed on the surface of granulocytes and monocytes and is upregulated during the differentiation of myeloid leukemia cells (2, 3). A motif in the ECD, which promotes dimerization of other JAM family proteins, is required for surface localization of AMICA (2). AMICA mediates adhesion of monocytes to endothelial cells (2) and neutrophil migration across epithelial cell monolayers (3). This latter function involves specific interactions of AMICA with CXADR in epithelial tight junctions (3). In particular, the membrane proximal Ig-like domain of AMICA binds the membrane-distal Ig-like domain of CXADR (3). AMICA does not appear to interact homophilically, as neutrophils adhere to immobilized CXADR but not to immobilized AMICA (3).

References

  1. Mandell, K.J. and C.A. Parkos (2005) Adv. Drug Deliv. Rev. 57:857.
  2. Moog-Lutz, C. et al. (2003) Blood 102:3371.
  3. Zen, K. et al. (2005) Mol. Biol. Cell 16:2694.

Long Name

Adhesion Molecule, Interacts With CXADR Antigen 1

Alternate Names

AMICA1, CREA7-1, JAML

Entrez Gene IDs

120425 (Human); 270152 (Mouse)

Gene Symbol

JAML

UniProt

Q80UL9

Additional AMICA/JAML Products

Product Documents for Mouse AMICA/JAML Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse AMICA/JAML Antibody

For research use only

Customer Reviews for Mouse AMICA/JAML Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Mouse AMICA/JAML Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 of 1 review Showing All
Filter By:
Clear Filters X
  • Mouse AMICA/JAML Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Mouse
    Verified Customer | Posted 12/13/2019
    Mouse AMICA/JAML Antibody AF3106

There are no reviews that match your criteria.

Showing  1 - 1 of 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies