Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Aminopeptidase P2/XPNPEP2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase P2/XPNPEP2
Tyr22-Ala650
Accession # Q91Y31

Specificity

Detects mouse Aminopeptidase P2/XPNPEP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross‑reactivity with recombinant human Aminopeptidase P2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Aminopeptidase P2/XPNPEP2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Aminopeptidase P2/XPNPEP2 (Catalog # 2494-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Aminopeptidase P2/XPNPEP2 (Catalog # 2494-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Aminopeptidase P2/XPNPEP2

The mouse XPNPEP2 gene encodes Aminopeptidase P2 (APP2), which is also known as X-prolyl Aminopeptidase 2 or membrane bound Aminopeptidase P (1, 2). It is a member of the M24 family of metalloproteases, which also contains methionine aminopeptidases, X-Pro dipeptidase, Aminopeptidase P1, Aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (3). Mammalian APP2 are predicted to be GPI-anchored membrane proteases and their biological functions have been reviewed (4). Mouse APP2 is expressed in many adult tissues and in embryos (5). The purified rmAPP2 corresponds to the ectodomain and is shown here to be an active aminopeptidase, removing an N-terminal amino acid from a peptide that contains a Pro residue at the second position.

References

  1. Kawai, J. et al. (2001) Nature 409:685.
  2. Shibata, K. et al. (2000) Genome Res. 10:1757.
  3. Barrett, A.J. et al. (2004) Handbook of Proteolytic Enzymes, Elsevier Academic Press, San Diego.
  4. Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 934, Elsevier Academic Press, San Diego.
  5. Ersahin C. et al. (2003) Arch. Biochem. Biophys. 417:131.

Alternate Names

XPNPEP2

Entrez Gene IDs

7512 (Human); 170745 (Mouse)

Gene Symbol

XPNPEP2

UniProt

Q91Y31

Additional Aminopeptidase P2/XPNPEP2 Products

Product Documents for Mouse Aminopeptidase P2/XPNPEP2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse Aminopeptidase P2/XPNPEP2 Antibody

The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for non-commercial research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. Inparticular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This Product is manufactured under a license to U.S. Patent Nos. 6,399,349 and 7,273,718.

Any party desiring rights under this patent should contact Ryogen LLC, Montebello Park, 75 Montebello Road, Suffern, NY 10901.

For research use only

Related Research Areas

Metalloproteases and Regulators

Customer Reviews for Mouse Aminopeptidase P2/XPNPEP2 Antibody

There are currently no reviews for this product. Be the first to review Mouse Aminopeptidase P2/XPNPEP2 Antibody and earn rewards!

Have you used Mouse Aminopeptidase P2/XPNPEP2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies