Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Neutralization, Immunocytochemistry, In vivo assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 185234
View all Artemin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Artemin
Ala112-Gly224
Accession # Q9Z0L2.1

Specificity

Detects mouse Artemin in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) AgRP, rmCripto, recombinant human (rh) Cripto-1, recombinant drosophila Dpp, recombinant rat GDNF, rhLAP, rhLatent TGF-beta 1, rhLefty A, rmLefty‑1, rmMIS, rhNeurturin, rmNodal, rhTGF-alpha, rhTGF-beta 1, rhTGF-beta 1.2, rhTGF-beta 2, rhTGF-beta 3, or recombinant Amphibian TGF-beta 5 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Artemin Antibody

Artemin in Human tonsil.

Artemin was detected in immersion fixed paraffin-embedded sections of human tonsil using Rat Anti-Mouse Artemin Monoclonal Antibody (Catalog # MAB10851) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes in germinal centers. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Mouse Artemin Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human tonsil

Western Blot

1 µg/mL
Sample: Recombinant Mouse Artemin (Catalog # 1085-AR)
under non-reducing conditions only

Reviewed Applications

Read 2 reviews rated 5 using MAB10851 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Artemin

Artemin is a member of the Glia Cell-Derived Neurotrophic factor (GDNF) family ligands, which include GDNF, Persephin, Artemin, and Neurturin. GDNF family ligands are distant members of the Transforming Growth Factor beta (TGF-beta ) superfamily (1‑4). Similar to other TGF-beta family proteins, Artemin is synthesized as a large precursor protein that is cleaved at the dibasic cleavage site (RXXR) to release the carboxy-terminal domain. The carboxy-terminal domain of Artemin contains the characteristic seven conserved cysteine residues necessary for the formation of the cysteine-knot and the single interchain disulfide bond. Biologically active Artemin is a disulfide-linked homodimer of the carboxy-terminal 113 amino acid residues. Mature mouse Artemin shares 88.5% amino acid sequence similarity with human Artemin. Mature Artemin also shares approximately 40% amino acid sequence identity with the other three members of the GDNF family ligands (5). Bioactivities of all GDNF family ligands are mediated through a receptor complex composed of a high affinity ligand binding component (GFR alpha 1‑GFR alpha 4) and a common signaling component, cRET (receptor tyrosine kinase) (5‑8). Artemin prefers to bind to GFR alpha 3 and activites the GFR alpha 3‑RET. However, in the presence of RET, it can bind to GFR alpha 1 as well (4, 5, 9). Artemin has been shown to promote the survival and growth of various peripheral and central neurons, including sympathetic and dopaminergic neurons. It may also play an important role in the development of sympathetic neurons and several organs (5, 10, 11).

References

  1. Lin, L-F.H. et al. (1993) Science 260:1130.
  2. Milbrandt, J. et al. (1998) Neuron 20:245.
  3. Kotzbauer, P.T. et al. (1996) Nature 384:467.
  4. Baloh, R.H. et al. (1998) Neuron 21:1291.
  5. Takahashi, M. (2001) Cytokine and Growth Factor Reviews 12:361.
  6. Baloh, R.H. et al. (1997) Neuron 18:793.
  7. Jing, S. et al. (1996) Cell 85:1113.
  8. Jing, S. et al. (1997) J Biol Chem 272:33111.
  9. Nishino, J. et al. (1999) Neuron 23:725.
  10. Enomoto, H. et al. (2001) Development 128:3963.
  11. Andres, R. et al. (2001) Development 128:3685.

Alternate Names

ARTN, Enovin, EVN, Neublastin

Entrez Gene IDs

9048 (Human); 11876 (Mouse)

Gene Symbol

ARTN

UniProt

Q9Z0L2.1

Additional Artemin Products

Product Documents for Mouse Artemin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Artemin Antibody

For research use only

Related Research Areas

GDNF Family

Citations for Mouse Artemin Antibody

Customer Reviews for Mouse Artemin Antibody (2)

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2 Customer Ratings
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Showing  1 - 2 of 2 reviews Showing All
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  • Mouse Artemin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Lung cancer tissue
    Species: Mouse
    Verified Customer | Posted 07/08/2022
    Mouse Artemin Antibody MAB10851
  • Mouse Artemin Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Endometrial carcinoma
    Species: Mouse
    Verified Customer | Posted 10/22/2021
    Mouse Artemin Antibody MAB10851

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