Mouse B220/CD45R Alexa Fluor® 405-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB1217V) or (B) Rat IgG2AAlexa Fluor 405 Isotype Control (Catalog # IC006V). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells. The level of B220 antigen expression on cells of the B cell lineage is developmentally regulated, and the antibody described here is commonly used to identify this B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses. The extracellular region of mouse B220 shares 41% amino acid sequence identity with the extracellular region of human CD45.
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