Mouse B220/CD45R Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1217G
Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry.
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Mouse B220/CD45R Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse B220/CD45R.
Source
Monoclonal Rat IgG2A Clone # RA3-6B2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1217G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry B220/CD45R  in Mouse Splenocytes. View Larger

B220/CD45R in Mouse Splenocytes. B220/CD45R was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B220/CD45R Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1217G; green) at 5 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: B220/CD45R

B220 (accession # P06800) is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

References
  1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
  2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
  3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
  4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
  5. Asensi, V.K. et al. (1989) Immunology 68:204.
  6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 24699 (Rat)
Alternate Names
B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide

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