Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Rat Anti-Mouse B220/CD45R Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1217G) or (B) Rat IgG2A Alexa Fluor 488 Isotype Control (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.
B220/CD45R in Mouse Splenocytes.
B220/CD45R was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse B220/CD45R Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1217G; green) at 5 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
B220 (accession # P06800) is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
Coffman, R.L. (1982) Immuno. Rev. 69:5.
Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
Allman, D.M. et al. (1992) J. Immunol. 149:2533.
Asensi, V.K. et al. (1989) Immunology 68:204.
Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Cluster of Differentiation 45
Entrez Gene IDs:
5788 (Human); 19264 (Mouse); 24699 (Rat)
B220; CD45 antigen; CD45R; EC 188.8.131.52; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
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