Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse B220/CD45R Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB1217T) or (B) Rat IgG2A Alexa Fluor 594 Isotype Control (Catalog # IC006T). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells. The level of B220 antigen expression on the B cell lineage is developmentally regulated and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses.
Cluster of Differentiation 45
Entrez Gene IDs:
5788 (Human); 19264 (Mouse); 24699 (Rat)
B220; CD45 antigen; CD45R; EC 22.214.171.124; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.