Mouse B220/CD45R Biotinylated Antibody Summary
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated B220/CD45R Monoclonal Antibody (Catalog # BAM1217) or (B) Rat IgG2A Biotinylated isotype control antibody (Catalog # IC006B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.
B220/CD45R in Mouse Splenocytes. B220/CD45R was detected in immersion fixed mouse splenocytes using Mouse B220/CD45R Biotinylated Monoclonal Antibody (Catalog # BAM1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
B220 (accession # P06800) is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
- Coffman, R.L. (1982) Immuno. Rev. 69:5.
- Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
- Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
- Allman, D.M. et al. (1992) J. Immunol. 149:2533.
- Asensi, V.K. et al. (1989) Immunology 68:204.
- Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Citations for Mouse B220/CD45R Biotinylated Antibody
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Citations: Showing 1 - 3
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Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology
Alzheimers Res Ther, 2016;8(1):54.
Sample Types: Whole Cells
Effects of a germ-free environment on gut immune regulation and diabetes progression in non-obese diabetic (NOD) mice.
Authors: Alam C, Bittoun E, Bhagwat D, Valkonen S, Saari A, Jaakkola U, Eerola E, Huovinen P, Hanninen A
Sample Types: Whole Cells
Applications: Flow Cytometry
Impaired salivary gland function in NOD mice: association with changes in cytokine profile but not with histopathologic changes in the salivary gland.
Authors: Jonsson MV, Delaleu N, Brokstad KA, Berggreen E, Skarstein K
Arthritis Rheum., 2006;54(7):2300-5.
Sample Types: Whole Tissue
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