Mouse B220/CD45R Biotinylated Antibody

  (3 citations)     
Datasheet / CoA / SDS
Product Details
Citations (3)
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse B220/CD45R. Reacts with the extracellular domain of mouse B220/CD45R antigen.
  • Source
    Monoclonal Rat IgG2A Clone # RA3-6B2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Abelson mouse leukemia virus-induced pre-B tumor cells
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • Immunoprecipitation
    Coffman, R.L. (1982) Immuno. Rev. 69:5.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated B220/CD45R Monoclonal Antibody (Catalog # BAM1217) or (B) Rat IgG2A Biotinylated isotype control antibody (Catalog # IC006B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.      
Immunocytochemistry
B220/CD45R in Mouse Splenocytes. B220/CD45R was detected in immersion fixed mouse splenocytes using Mouse B220/CD45R Biotinylated Monoclonal Antibody (Catalog # BAM1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
      12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Background: B220/CD45R
B220 (accession # P06800) is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1-3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2-4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
  • References:
    1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
    2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
    3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
    4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
    5. Asensi, V.K. et al. (1989) Immunology 68:204.
    6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
  • Long Name:
    Cluster of Differentiation 45
  • Entrez Gene IDs:
    5788 (Human); 19264 (Mouse); 24699 (Rat)
  • Alternate Names:
    B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology
    Alzheimers Res Ther, 2016;8(1):54.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  2. Effects of a germ-free environment on gut immune regulation and diabetes progression in non-obese diabetic (NOD) mice.
    Authors: Alam C, Bittoun E, Bhagwat D, Valkonen S, Saari A, Jaakkola U, Eerola E, Huovinen P, Hanninen A
    Diabetologia, 2011;54(6):1398-406.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Impaired salivary gland function in NOD mice: association with changes in cytokine profile but not with histopathologic changes in the salivary gland.
    Authors: Jonsson MV, Delaleu N, Brokstad KA, Berggreen E, Skarstein K
    Arthritis Rheum., 2006;54(7):2300-5.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen

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