Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated B220/CD45R Monoclonal Antibody (Catalog # BAM1217) or (B) Rat IgG2A Biotinylated isotype control antibody (Catalog # IC006B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.
B220/CD45R in Mouse Splenocytes.
B220/CD45R was detected in immersion fixed mouse splenocytes using Mouse B220/CD45R Biotinylated Monoclonal Antibody (Catalog # BAM1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
B220 (accession # P06800) is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1-3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2-4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).
Coffman, R.L. (1982) Immuno. Rev. 69:5.
Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
Allman, D.M. et al. (1992) J. Immunol. 149:2533.
Asensi, V.K. et al. (1989) Immunology 68:204.
Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Cluster of Differentiation 45
Entrez Gene IDs:
5788 (Human); 19264 (Mouse); 24699 (Rat)
B220; CD45 antigen; CD45R; EC 18.104.22.168; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
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