Mouse B220/CD45R Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM1217

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Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry.
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Citations (3)
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Mouse B220/CD45R Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse B220/CD45R. Reacts with the extracellular domain of mouse B220/CD45R antigen.
Source
Monoclonal Rat IgG2A Clone # RA3-6B2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Abelson mouse leukemia virus-induced pre-B tumor cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunoprecipitation
Coffman, R.L. (1982) Immuno. Rev. 69:5.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of B220/CD45R antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with (A) Rat Anti-Mouse Biotinylated B220/CD45R Monoclonal Antibody (Catalog # BAM1217) or (B) Rat IgG2A Biotinylated isotype control antibody (Catalog # IC006B) followed by Streptavidin-Phycoerythrin (Catalog # F0040) and Rat Anti-Mouse CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry B220/CD45R antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

B220/CD45R in Mouse Splenocytes. B220/CD45R was detected in immersion fixed mouse splenocytes using Mouse B220/CD45R Biotinylated Monoclonal Antibody (Catalog # BAM1217) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B220/CD45R

B220 (accession # P06800) is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells (1‑3). The level of B220 antigen expression on the B cell lineage is developmentally regulated (2‑4) and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses (5, 6).

References
  1. Coffman, R.L. (1982) Immuno. Rev. 69:5.
  2. Hardy, R.R. et al. (1991) J. Exp. Med. 173:1213.
  3. Hathcock, K.S. et al. (1992) J. Immunol. 149:2286.
  4. Allman, D.M. et al. (1992) J. Immunol. 149:2533.
  5. Asensi, V.K. et al. (1989) Immunology 68:204.
  6. Domiati-Saad, R. et al. (1993) J. Immunol. 151:5936.
Long Name
Cluster of Differentiation 45
Entrez Gene IDs
5788 (Human); 19264 (Mouse); 24699 (Rat)
Alternate Names
B220; CD45 antigen; CD45R; EC 3.1.3.48; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide

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Citations for Mouse B220/CD45R Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology
    Alzheimers Res Ther, 2016-12-15;8(1):54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Effects of a germ-free environment on gut immune regulation and diabetes progression in non-obese diabetic (NOD) mice.
    Authors: Alam C, Bittoun E, Bhagwat D, Valkonen S, Saari A, Jaakkola U, Eerola E, Huovinen P, Hanninen A
    Diabetologia, 2011-03-05;54(6):1398-406.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Impaired salivary gland function in NOD mice: association with changes in cytokine profile but not with histopathologic changes in the salivary gland.
    Authors: Jonsson MV, Delaleu N, Brokstad KA, Berggreen E, Skarstein K
    Arthritis Rheum., 2006-07-01;54(7):2300-5.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr

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