Mouse B220/CD45R Alexa Fluor® 647-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse B220/CD45R.
  • Source
    Monoclonal Rat IgG2A Clone # RA3-6B2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Abelson mouse leukemia virus-induced pre-B tumor cells
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of B220/CD45R in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE‑conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse B220/CD45R Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB1217R) or (B) Rat IgG2A Alexa Fluor 647 Isotype Control (Catalog # IC006R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: B220/CD45R
B220 is the mouse CD45 isoform CD45R, which is predominantly expressed on all B lymphocytes, including pro-, mature and activated B cells. The level of B220 antigen expression on the B cell lineage is developmentally regulated and this antibody is commonly used as a B cell marker. RA3-6B2 has been reported to inhibit in vivo B cell responses.
  • Long Name:
    Cluster of Differentiation 45
  • Entrez Gene IDs:
    5788 (Human); 19264 (Mouse); 24699 (Rat)
  • Alternate Names:
    B220; CD45 antigen; CD45R; EC; L-CA; LY5; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 647-conjugated Antibody

Ctrl IC006R
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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