Mouse B7-1/CD80 Antibody

Catalog # Availability Size / Price Qty
MAB740
MAB740-SP
B7-1/CD80 in Mouse Colon Tissue.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)

Mouse B7-1/CD80 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse B7-1/CD80 in direct ELISAs and Western blots. No cross-reactivity with recombinant mouse (rm) rmB7-2, rmB7-H2 and recombinant human (rh) B7-H1.
Source
Monoclonal Rat IgG2A Clone # 111114
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-1/CD80
Val38-Asn246
Accession # Q00609
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunohistochemistry
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry B7-1/CD80 antibody in Mouse Colon Tissue by Immunohistochemistry (IHC-P). View Larger

B7-1/CD80 in Mouse Colon Tissue. B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of mouse colon tissue using Rat Anti-Mouse B7-1/CD80 Monoclonal Antibody (Catalog # MAB740) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry B7-1/CD80 antibody in Human Tonsil by Immunohistochemistry (IHC-P). View Larger

B7‑1/CD80 in Human Tonsil. B7-1/CD80 was detected in immersion fixed paraffin-embedded sections of human tonsil using Rat Anti-Mouse B7-1/CD80 Monoclonal Antibody (Catalog # MAB740) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocyte cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-1/CD80

B7-1 and B7-2, together with their receptors CD28 and CTLA-4, constitute one of the dominant costimulatory pathways that regulate T and B cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells. Additionally, B7-2 is expressed at low levels on monocytes and can be up-regulated through interferon gamma. B7-1 and B7-2 are both members of the immunoglobulin superfamily. Mouse B7-1 is a 306 amino acid (aa) protein containing a putative 37 aa signal peptide, a 190 aa extracellular domain, a 22 aa transmembrane domain, and a 38 aa cytoplasmic domain. Mouse B7-1 and B7-2 share 28% amino acid identity. Mouse and human B7-1 share 44% amino acid identity. However, it has been observed that both human and mouse B7‑1 and B7‑2 can bind to either human or mouse CD28 and CTLA-4, suggesting that there are conserved amino acids which form the B7-1/B7-2/CD28/CTLA-4 critical binding sites.

References
  1. Azuma, M. et al. (1993) Nature 366:76.
  2. Freeman, G.J. et al. (1993) Science 262:909.
  3. Freeman, G. et al. (1991) J. Exp. Med. 174:625.
  4. Selvakumar, A. et al. (1993) Immunogenetics 38:292.
  5. Chen, C. et al. (1994) J. Immunol. 152:4929.
  6. Freeman, G.J. et al. (1993) J. Exp. Med. 178:2185.
Entrez Gene IDs
941 (Human); 12519 (Mouse); 25408 (Rat); 102140078 (Cynomolgus Monkey)
Alternate Names
Activation B7-1 antigen; B7; B71; B7-1; BB1; CD28LG1; CD28LGB7-1 antigen); CD80 antigen; CD80 molecule; CD80; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; T-lymphocyte activation antigen CD80

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Citations for Mouse B7-1/CD80 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Macrophage polarity and wound age determination
    Authors: Y Kuninaka, Y Ishida, A Ishigami, M Nosaka, J Matsuki, H Yasuda, A Kofuna, A Kimura, F Furukawa, T Kondo
    Scientific Reports, 2022-11-25;12(1):20327.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Cystatin C Plays a Sex-Dependent Detrimental Role in Experimental Autoimmune Encephalomyelitis
    Authors: V Hoghooghi, AL Palmer, A Frederick, Y Jiang, JE Merkens, A Balakrishn, TM Finlay, A Grubb, E Levy, P Gordon, FR Jirik, MD Nguyen, C Schuurmans, F Visser, SE Dunn, SS Ousman
    Cell Rep, 2020-10-06;33(1):108236.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. Mechanistic dissection of the PD-L1:B7-1 co-inhibitory immune complex
    Authors: SC Garrett-Th, A Massimi, EV Fedorov, JB Bonanno, L Scandiuzzi, B Hillerich, RD Seidel, JD Love, SJ Garforth, C Guha, SC Almo
    PLoS ONE, 2020-06-04;15(6):e0233578.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  4. Interleukin-12p35 Knock Out Aggravates Doxorubicin-Induced Cardiac Injury and Dysfunction by Aggravating the Inflammatory Response, Oxidative Stress, Apoptosis and Autophagy in Mice
    Authors: J Ye, Y Huang, B Que, C Chang, W Liu, H Hu, L Liu, Y Shi, Y Wang, M Wang, T Zeng, W Zhen, Y Xu, L Shi, J Liu, H Jiang, D Ye, Y Lin, J Wan, Q Ji
    EBioMedicine, 2018-09-15;35(0):29-39.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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Mouse B7-1/CD80 Antibody
By Anonymous on 10/22/2021
Application: IHC Sample Tested: Mucosa Species: Mouse