Mouse B7-H2 Antibody

Catalog # Availability Size / Price Qty
MAB10083-100
MAB10083-SP
ICOS binding to Mouse B7-H2-transfected HEK293 Human Cell Line is Blocked by Mouse B7-H2 Antibody.
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Mouse B7-H2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse B7-H2 in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 987602
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-H2
Met1-Lys279
Accession # Q9JHJ8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Blockade of Receptor-ligand Interaction
0.5 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize B7-H2-induced IL-4 secretion in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.3-1.8 μg/mL in the presence of 2.5 ng/mL Recombinant Mouse B7-H2 and Rat Anti-Mouse CD3 Monoclonal Antibody.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Blockade of Receptor-ligand Interaction ICOS binding to Mouse B7-H2-transfected HEK293 Human Cell Line is Blocked by Mouse B7-H2 Antibody. View Larger

ICOS binding to Mouse B7-H2-transfected HEK293 Human Cell Line is Blocked by Mouse B7-H2 Antibody. In a functional flow cytometry test, Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant mouse B7-H2 (black dotted line). Binding is completely blocked (orange histogram) by 0.5 μg/mL of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). Rat IgG2A Isotype Control (Catalog # MAB006) at 0.5 μg/mL was used as a control (blue line). Ligand binding was detected by staining cells with Mouse Anti-Human IgG Fc APC‑conjugated Monoclonal Antibody (Catalog # FAB110A).

Flow Cytometry Detection of Mouse B7-H2 in HEK293 Human Cell Line Transfected with Mouse B7-H2 and eGFP by Flow Cytometry. View Larger

Detection of Mouse B7-H2 in HEK293 Human Cell Line Transfected with Mouse B7-H2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse B7-H2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083) followed by APC-conjugated Anti-Rat IgG Secondary Antibody(Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins.

Neutralization IL-4 Secretion Induced by B7-H2 and Neutralization by Mouse B7-H2 Antibody. View Larger

IL-4 Secretion Induced by B7-H2 and Neutralization by Mouse B7-H2 Antibody. Recombinant Mouse B7-H2 (Catalog # 8127-B7) induces IL-4 secretion in the D10.G4.1 mouse helper T cell line in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) in a dose-dependent manner (orange line), as measured by the Mouse IL-4 Quantikine ELISA Kit (Catalog # M4000B). Under these conditions, IL-4 secretion elicited by B7-H2 (2.5 μg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). The ND50 is typically 0.3-1.8 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: B7-H2

B7-H2, also called B7RP-1 and ICOSL, is a 60 kDa member of the B7 family of immune costimulatory proteins. It is expressed on resting B cells, dendritic cells, monocytes, alveolar epithelial cells, and placental trophoblasts. B7-H2 interactions with ICOS promote lymphocyte differentiation and activation. Within the ECD, mouse B7-H2 shares 49% and 70% aa sequence identity with human and rat B7-H2, respectively.

Long Name
B7 Homolog 2
Entrez Gene IDs
23308 (Human); 50723 (Mouse); 499415 (Rat); 102132485 (Cynomolgus Monkey)
Alternate Names
B7H2; B7-H2; B7H2B7 homolog 2; B7-H2B7-related protein 1; B7RP-1; B7RP1B7-like protein Gl50; B7RP-1LICOS; CD275 antigen; CD275; GL50; GL50transmembrane protein B7-H2 ICOS ligand; ICOSL; ICOS-L; ICOSLG; inducible T-cell co-stimulator ligand; KIAA0653ICOS ligand

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