B7-H2, also called B7RP-1 and ICOSL, is a 60 kDa member of the B7 family of immune costimulatory proteins. It is expressed on resting B cells, dendritic cells, monocytes, alveolar epithelial cells, and placental trophoblasts. B7-H2 interactions with ICOS promote lymphocyte differentiation and activation. Within the ECD, mouse B7-H2 shares 49% and 70% aa sequence identity with human and rat B7-H2, respectively.
Key Product Details
Species Reactivity
Mouse
Applications
Blockade of Receptor-ligand Interaction, Neutralization, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 987602
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse B7-H2
Met1-Lys279
Accession # Q9JHJ8
Met1-Lys279
Accession # Q9JHJ8
Specificity
Detects mouse B7-H2 in direct ELISAs.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse B7‑H2 Antibody
ICOS binding to Mouse B7-H2-transfected HEK293 Human Cell Line is Blocked by Mouse B7-H2 Antibody.
In a functional flow cytometry test, Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant mouse B7-H2 (black dotted line). Binding is completely blocked (orange histogram) by 0.5 µg/mL of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). Rat IgG2A Isotype Control (Catalog # MAB006) at 0.5 µg/mL was used as a control (blue line). Ligand binding was detected by staining cells with Mouse Anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A).
Detection of Mouse B7-H2 in HEK293 Human Cell Line Transfected with Mouse B7-H2 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with (A) mouse B7-H2 or (B) irrelevant transfectants and eGFP was stained with Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006). View our protocol for Staining Membrane-associated Proteins.
IL-4 Secretion Induced by B7-H2 and Neutralization by Mouse B7-H2 Antibody.
Recombinant Mouse B7-H2 (Catalog # 8127-B7) induces IL-4 secretion in the D10.G4.1 mouse helper T cell line in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) in a dose-dependent manner (orange line), as measured by the Mouse IL-4 Quantikine ELISA Kit (Catalog # M4000B). Under these conditions, IL-4 secretion elicited by B7-H2 (2.5 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse B7-H2 Monoclonal Antibody (Catalog # MAB10083). The ND50 is typically 0.3-1.8 µg/mL.Applications for Mouse B7‑H2 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
0.5 µg/106 cells
Sample: In a functional flow cytometry test, 0.5 μg/mL of Rat Anti-Mouse B7-H2 Antibody(Catalog # MAB10083) will block the binding of 500 ng/mL Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS) to HEK293 human embryonic kidney cell line transfected with recombinant mouse B7-H2.
Sample: In a functional flow cytometry test, 0.5 μg/mL of Rat Anti-Mouse B7-H2 Antibody(Catalog # MAB10083) will block the binding of 500 ng/mL Recombinant Mouse ICOS Fc Chimera (Catalog # 168-CS) to HEK293 human embryonic kidney cell line transfected with recombinant mouse B7-H2.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Mouse B7-H2 and eGFP
Sample: HEK293 Human Cell Line Transfected with Mouse B7-H2 and eGFP
Neutralization
Measured by its ability to neutralize B7-H2-induced IL-4 secretion in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.3-1.8 μg/mL in the presence of 2.5 ng/mL Recombinant Mouse B7-H2 and Rat Anti-Mouse CD3 Monoclonal Antibody.
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
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- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: B7-H2
Long Name
B7 Homolog 2
Alternate Names
B7H2, B7RP-1, CD275, GL50, ICOSL, ICOSLG
Gene Symbol
ICOSLG
UniProt
Additional B7-H2 Products
Product Documents for Mouse B7‑H2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse B7‑H2 Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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