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Mouse BAFF / BLyS / TNFSF13B ELISA Standard Curve
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Citations (8)
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Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
62.5 - 4,000 pg/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse BAFF / BLyS / TNFSF13B. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Data Example

Mouse BAFF / BLyS / TNFSF13B ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: BAFF/BLyS/TNFSF13B

BAFF (B-cell activating factor), also known as BLyS, TALL-1, THANK, and TNFSF13B, is a TNF superfamily ligand that plays a critical role in the development and survival of B lineage cells. It can be expressed as a homotrimer or as a heteromer in association with the related protein APRIL. It is produced by many hematopoietic cell types, and a soluble form can be released by activated neutrophils. Both BAFF and APRIL signal through the receptors BCMA and TACI, and BAFF additionally signals through BAFF R. Mice that overexpress BAFF exhibit elevated B cell numbers, increased formation and size of germinal centers, and symptoms of autoimmunity. In addition, BAFF costimulates T cell activation, promotes a Th1 biased immune response, and promotes the expansion of Treg cells.

Long Name:
B cell Activating Factor
Entrez Gene IDs:
10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names:
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4

Citations for Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Circulating CD138 enhances disease progression by augmenting autoreactive antibody production in a mouse model of systemic lupus erythematosus
    Authors: L Liu, M Akkoyunlu
    The Journal of Biological Chemistry, 2021;0(0):101053.
    Species: Mouse
    Sample Types: Serum
  2. B cell activating factor (BAFF) from neutrophils and dendritic cells is required for protective B cell responses against Salmonella typhimurium infection
    Authors: R Kuley, KE Draves, DH Fuller, NV Giltiay, EA Clark, D Giordano
    PLoS ONE, 2021;16(10):e0259158.
    Species: Mouse
    Sample Types: Serum
  3. Variable dependency on BAFF in IgG antibody production during Leishmania infection
    Authors: N Azuma, S Omachi, W Hanazawa, A Morimoto, C Sanjoba, Y Matsumoto, W Fujii, Y Goto
    Parasitol. Int., 2019;74(0):101997.
    Species: Mouse
    Sample Types: Serum
  4. ORMDL3 Facilitates the Survival of Splenic B Cells via an ATF6?-Endoplasmic Reticulum Stress-Beclin1 Autophagy Regulatory Pathway
    Authors: J Dang, X Bian, X Ma, J Li, F Long, S Shan, Q Yuan, Q Xin, Y Li, F Gao, Y Gong, Q Liu
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Serum
  5. B-cell activating factor deficiency suppresses splenomegaly during Leishmania donovani infection
    Authors: S Omachi, W Fujii, N Azuma, A Morimoto, C Sanjoba, Y Matsumoto, Y Goto
    Biochem. Biophys. Res. Commun., 2017;0(0):.
    Species: Mouse
    Sample Types: Serum
  6. Silica-Triggered Autoimmunity in Lupus-Prone Mice Blocked by Docosahexaenoic Acid Consumption
    PLoS ONE, 2016;11(8):e0160622.
    Species: Mouse
    Sample Types: BALF
  7. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2016;30(1):163-72.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. B cell homeostasis and follicle confines are governed by fibroblastic reticular cells.
    Authors: Cremasco, Viviana, Woodruff, Matthew, Onder, Lucas, Cupovic, Jovana, Nieves-Bonilla, Janice M, Schildberg, Frank A, Chang, Jonathan, Cremasco, Floriana, Harvey, Christop, Wucherpfennig, Kai, Ludewig, Burkhard, Carroll, Michael, Turley, Shannon
    Nat Immunol, 2014;15(10):973-81.
    Species: Mouse
    Sample Types: Serum


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Reviews for Mouse BAFF/BLyS/TNFSF13B DuoSet ELISA

Average Rating: 5 (Based on 2 Reviews)

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By Anonymous on 08/10/2019
Sample Tested: Cell Lysates

By Anonymous on 10/08/2018
Sample Tested: Serum