Mouse beta -NGF Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB11562-100
MAB11562-SP
Proliferation Induced by  beta -NGF and Neutralization by Mouse  beta ‑NGF Antibody.
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Mouse beta -NGF Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse beta ‑NGF in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2529C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived mouse beta ‑NGF
Ser122-Gly241
Accession # Q6LDU8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line.The Neutralization Dose (ND50) is typically 0.05-0.5 μg/ml in the presence of 5 ng/mL Recombinant Mouse beta ‑NGF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization View Larger

Proliferation Induced by beta -NGF and Neutralization by Mouse beta ‑NGF Antibody. Recombinant Mouse beta -NGF (1156-NG) induces proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Rezazurin (AR002). Proliferation elicited by Recombinant Mouse beta -NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Mouse beta -NGF Monoclonal Antibody (Catalog # MAB11562). The ND50 is typically 0.05-0.5 µg/ml.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-NGF

NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system (1). In addition, beta -NGF can act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons (2).  beta -NGF has also been shown to have biological effects on a variety of neurons, glia, and nonneural cells (3). NGF was initially isolated from the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF, exhibits all the biological activities ascribed to NGF (4 - 5). Recombinant mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF (6).

NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). NT-6 and NT-7 were also identified in fish (7, 8). Biological activities of the NGF family are mediated through binding and activation of two types of receptors, TrKs (TrKA, TrKB, and TrKC) and NGF receptor. TrKs are single-pass membrane proteins belonging to the receptor tyrosine kinase family. The neurotrophins bind to TrKs specifically. NGF is specific for TrKA, BDNF and NT-4 for TrKB, and NT-3 mostly for TrKC. NGF receptor is a TNF receptor superfamily protein that binds to all the neurotrophins with similar affinity (9). TrkA is often referred to as the high-affinity receptor. However, most high affinity binding is mediated by the interaction of NGF receptor with TrKA. Both NGF and its receptors are expressed during development, adult life and aging in many cell types in CNS and PNS, immune and inflammatory systems. NGF signaling has been shown to play an important role in neuroprotection and repair (3, 10).

References
  1. Levi-Montalcini, R and V. Hamberger (1951) J. Exp. Zool. 116:321.
  2. Levi-Montacini, R. Ann. Rev. Neurosci. 5:341.
  3. Sofroniew, M.V. et al. (2001) Ann Rev Neurosci. 24:1217.
  4. Edwards, R.H. et al. (1988) J Biol Chem. 263:6810.
  5. Angeletti, R H. et al. (1973) Biochemistry 12:100.
  6. Ullrich, A. et al. (1983) Nature 303:821.
  7. Gotz, R. et al. (1994) Nature 372:266.
  8. Lai, K.O. et al. (1998) Mol. Cell. Neurosci. 11:64.
  9. Bothwell, M. (1995) Ann Rev Neurosci. 18:223.
  10. E.J. Huang and L.F. Reichardt (2001) Ann Rev Neurosci. 24:677.
Long Name
beta Nerve Growth Factor
Entrez Gene IDs
4803 (Human); 18049 (Mouse); 310738 (Rat); 403402 (Canine); 103350089 (Rabbit)
Alternate Names
Beta nerve growth factor precursor; beta-nerve growth factor; betaNGF; beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428

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