Mouse/Canine Integrin  beta 2/CD18 Antibody

Catalog # Availability Size / Price Qty
AF2618
AF2618-SP
Detection of Integrin  beta 2/CD18 in Canine PBMCs by Flow Cytometry.
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Product Details
Citations (2)
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Mouse/Canine Integrin  beta 2/CD18 Antibody Summary

Species Reactivity
Mouse, Canine
Specificity
Detects mouse Integrin beta 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross‑reactivity with recombinant human Integrin beta 2 and recombinant mouse (rm) Integrin beta 6 is observed and less than 5% cross‑reactivity with rmIntegrin beta 1, rmIntegrin beta 4, and rmIntegrin beta 7 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 2
Gln24-Asn702
Accession # P11835
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Integrin  beta 2/CD18
Flow Cytometry
2.5 µg/106 cells
Mouse lymph node cells and canine peripheral blood mononuclear cells (PBMCs)
Adhesion Activation
The adhesion of CD3+ purified mouse T cells to immobilized Recombinant Mouse ICAM‑1 Fc Chimera (Catalog # 796-IC, 10 µg/mL, 100 µL/well) was maximally enhanced (2-2.5 fold) by 1 µg/mL of the antibody.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Adhesion Activation Detection of Integrin  beta 2/CD18 antibody in Canine PBMCs antibody by Flow Cytometry. View Larger

Detection of Integrin beta 2/CD18 in Canine PBMCs by Flow Cytometry. Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Mouse/Canine Integrin beta 2/CD18 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2618, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 2/CD18

Mouse Integrin beta 2 is a transmembrane glycoprotein with an 85 kDa molecular weight (predicted) and is expressed by all leukocytes. The Integrin beta chain can be found in heterodimeric complexes with Integrin subunits alpha D, alpha L (complex also known as LFA‑1), alpha M (Mac-1), or alpha X (CR-4) leading to different Integrin proteins. The extra cellular domains of mouse and canine Integrin beta 2 share an 80.3% aa sequence identity. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling and leukocyte trafficking.

Entrez Gene IDs
3689 (Human); 16414 (Mouse); 309684 (Rat); 101925124 (Cynomolgus Monkey)
Alternate Names
959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; Integrin beta 2; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta

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Citations for Mouse/Canine Integrin  beta 2/CD18 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. LFA-1/ICAM-1 Adhesion Pathway Mediates the Homeostatic Migration of Lymphocytes from Peripheral Tissues into Lymph Nodes through Lymphatic Vessels
    Authors: Guo, J;Xu, Z;Gunderson, RC;Xu, B;Michie, SA;
    Biomolecules
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Biomimetic Nanoparticles as a Theranostic Tool for Traumatic Brain Injury
    Authors: Assaf Zinger, Sirena Soriano, Gherardo Baudo, Enrica De Rosa, Francesca Taraballi, Sonia Villapol
    Advanced Functional Materials

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