Mouse CCL11/Eotaxin Antibody

(5 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL11/Eotaxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human (rh) CCL11/Eotaxin, recombinant mouse CCL7, and rhCCL21 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 42285
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CCL11/Eotaxin
    His24-Pro97
    Accession # P48298
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the Y3 rat myeloid cell line transfected with human CCR3. The Neutralization Dose (ND50) is typically 3-30 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse CCL11/Eotaxin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Mouse CCL11/Eotaxin Antibody. Recombinant Mouse CCL11/
Eotaxin (Catalog # 420‑ME) chemoattracts the Y3 rat myeloid cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by MTT staining. Chemotaxis elicited by Recombinant Mouse CCL11/
Eotaxin (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti‑Mouse CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB420). The ND50 is typically 3‑30 µg/mL.
Immunohistochemistry
CCL11/Eotaxin in Rat Intestine. CCL11/Eotaxin was detected in perfusion fixed frozen sections of rat intestine using Rat Anti-Mouse CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB420) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to fibroblasts in submucosae. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL11/Eotaxin

CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Mouse CCL11 cDNA encodes a 97 amino acid (aa) precursor protein from which the amino-terminal 23 aa are cleaved to generate the 74 aa mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high aa sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has been identified as a specific human CCL11 receptor.

  • References:
    1. Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
    2. Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
    3. Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
    4. Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
  • Entrez Gene IDs:
    6356 (Human); 20292 (Mouse)
  • Alternate Names:
    C-C motif chemokine 11; CCL11; chemokine (C-C motif) ligand 11; Eosinophil chemotactic protein; Eotaxin; eotaxin-1; MGC22554; SCYA11Small-inducible cytokine A11; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. CCL11 promotes migration and proliferation of mouse neural progenitor cells
    Authors: F Wang, N Baba, Y Shen, T Yamashita, E Tsuru, M Tsuda, N Maeda, Y Sagara
    Stem Cell Res Ther, 2017;8(1):26.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. Neutralization of regulated on activation, normal T cell expressed and secreted and eotaxin prevents the loss of dopaminergic neurons in a mouse model of Parkinson's disease
    J Biol Chem, 2016;0(0):.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  3. Reciprocal expression of IL-25 and IL-17A is important for allergic airways hyperreactivity.
    Authors: Barlow JL, Flynn RJ, Ballantyne SJ, McKenzie AN
    Clin. Exp. Allergy, 2011;41(10):1447-55.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  4. The ageing systemic milieu negatively regulates neurogenesis and cognitive function.
    Authors: Villeda SA, Luo J, Mosher KI, Zou B, Britschgi M, Bieri G, Stan TM, Fainberg N, Ding Z, Eggel A, Lucin KM, Czirr E, Park JS, Couillard-Despres S, Aigner L, Li G, Peskind ER, Kaye JA, Quinn JF, Galasko DR, Xie XS, Rando TA, Wyss-Coray T
    Nature, 2011;477(7362):90-4.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  5. IL-23 and Th17 cells enhance Th2-cell-mediated eosinophilic airway inflammation in mice.
    Authors: Wakashin H, Hirose K, Maezawa Y, Kagami S, Suto A, Watanabe N, Saito Y, Hatano M, Tokuhisa T, Iwakura Y, Puccetti P, Iwamoto I, Nakajima H
    Am. J. Respir. Crit. Care Med., 2008;178(10):1023-32.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
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