Mouse CCL19/MIP-3 beta DuoSet ELISA

Catalog # Availability Size / Price Qty
DY440
Ancillary Products Available
Mouse CCL19 / MIP-3 beta ELISA Standard Curve
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Product Details
Procedure
Citations (12)
FAQs
Supplemental Products
Reviews (2)

Mouse CCL19/MIP-3 beta DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Sufficient Materials
For fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse CCL19/MIP-3beta. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

The components listed above may be purchased separately:

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4

Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990)

Plate Sealers: ELISA Plate Sealers (Catalog # DY992)

Data Example

Mouse CCL19 / MIP-3 beta ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL19/MIP-3 beta

Macrophage inflammatory protein 3 beta (MIP-3 beta), also known as ELC (EBI1-ligand chemokine) and exodus-3, is constitutively expressed in various lymphoid tissues, including thymus, lymph nodes, appendix and spleen. It is distantly related to other CC chemokines (20-30% amino acid sequence identity).

Entrez Gene IDs:
6363 (Human); 24047 (Mouse); 362506 (Rat)
Alternate Names:
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

Assay Procedure

GENERAL ELISA PROTOCOL

Plate Preparation

  1. Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
  2. Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
  3. Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
  4. Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.

Assay Procedure

  1. Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
  2. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  3. Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
  4. Repeat the aspiration/wash as in step 2 of Plate Preparation.
  5. Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  6. Repeat the aspiration/wash as in step 2.
  7. Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
  8. Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
  9. Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.

Citations for Mouse CCL19/MIP-3 beta DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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  1. IL-7 and CCL19 expression in CAR-T cells improves immune cell infiltration and CAR-T cell survival in the tumor
    Authors: K Adachi, Y Kano, T Nagai, N Okuyama, Y Sakoda, K Tamada
    Nat. Biotechnol., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  2. Expression of the B cell differentiation factor BAFF and chemokine CXCL13 in a murine model of Respiratory Syncytial Virus infection
    Authors: W Alturaiki, AJ McFarlane, K Rose, R Corkhill, PS McNamara, J Schwarze, BF Flanagan
    Cytokine, 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. Cytotoxic chemotherapy reduces T cell trafficking to the spleen by downregulating the expression of C-C motif chemokine ligand 21 and C-C motif chemokine ligand 19
    Authors: L Liu, L Zhao, Y Yang, J Gao, C Hu, B Guo, B Zhu
    Oncol Lett, 2018;16(4):5013-5019.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Deletion of the Chemokine Binding Protein Gene from the Parapoxvirus Orf Virus Reduces Virulence and Pathogenesis in Sheep
    Authors: SB Fleming, C McCaughan, Z Lateef, A Dunn, LM Wise, NC Real, AA Mercer
    Front Microbiol, 2017;8(0):46.
    Species: Mouse
    Sample Types: Protein
  5. CCRL1/ACKR4 is expressed in key thymic microenvironments but is dispensable for T lymphopoiesis at steady state in adult mice.
    Authors: Lucas B, White A, Ulvmar M, Nibbs R, Sitnik K, Agace W, Jenkinson W, Anderson G, Rot A
    Eur J Immunol, 2015;45(2):574-83.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. De novo-induced self-antigen-specific Foxp3+ regulatory T cells impair the accumulation of inflammatory dendritic cells in draining lymph nodes.
    Authors: Alissafi T, Hatzioannou A, Ioannou M, Sparwasser T, Grun J, Grutzkau A, Verginis P
    J Immunol, 2015;194(12):5812-24.
    Species: Mouse
    Sample Types: Tissue Homogenates
  7. Immunization with HSV-2 gB-CCL19 Fusion Constructs Protects Mice against Lethal Vaginal Challenge.
    Authors: Yan Y, Hu K, Deng X, Guan X, Luo S, Tong L, Du T, Fu M, Zhang M, Liu Y, Hu Q
    J Immunol, 2015;195(1):329-38.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. The B lymphocyte differentiation factor (BAFF) is expressed in the airways of children with CF and in lungs of mice infected with Pseudomonas aeruginosa.
    Authors: Neill, Daniel R, Saint, Gemma L, Bricio-Moreno, Laura, Fothergill, Joanne L, Southern, Kevin W, Winstanley, Craig, Christmas, Stephen, Slupsky, Joseph R, McNamara, Paul S, Kadioglu, Aras, Flanagan, Brian F
    PLoS ONE, 2014;9(5):e95892.
    Species: Mouse
    Sample Types: Tissue Homogenates
  9. Co-delivery of ccl19 gene enhances anti-caries DNA vaccine pCIA-P immunogenicity in mice by increasing dendritic cell migration to secondary lymphoid tissues.
    Authors: Yan Y, Qi S, Su L, Xu Q, Fan M
    Acta Pharmacol Sin, 2013;34(3):432-40.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  10. CCR2 plays a critical role in dendritic cell maturation: possible role of CCL2 and NF-kappa B.
    Authors: Jimenez F, Quinones MP, Martinez HG, Estrada CA, Clark K, Garavito E, Ibarra J, Melby PC, Ahuja SS
    J. Immunol., 2010;184(10):5571-81.
    Species: Mouse
    Sample Types: Tissue Homogenates
  11. CCR7 modulates pulmonary and lymph node inflammatory responses in cigarette smoke-exposed mice.
    Authors: Demoor T, Bracke KR, Vermaelen KY, Dupont L, Joos GF, Brusselle GG
    J. Immunol., 2009;183(12):8186-94.
    Species: Mouse
    Sample Types: BALF
  12. Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
    Authors: Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
    J. Immunol., 2008;181(9):6189-200.
    Species: Mouse
    Sample Types: Cell Culture Supernates

FAQs

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Reviews for Mouse CCL19/MIP-3 beta DuoSet ELISA

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Mouse CCL19/MIP-3 beta DuoSet ELISA
By Anonymous on 05/23/2016
Sample Tested: Tissue Homogenates

Mouse CCL19/MIP-3 beta DuoSet ELISA
By Anonymous on 05/23/2016
Sample Tested: Tissue Homogenates