Mouse CCL21/6Ckine Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CCL21/6Ckine in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) 6Ckine, rhMIP-1 alpha, rhMIP-1 beta, recombinant mouse (rm) MIP-1 alpha, rmMIP-1 beta, rhMIP-1δ, rmMIP-1 gamma, rmMIP-2, rhMIP-3 alpha, rmMIP-3 alpha, rhMIP-3 beta, rmMIP-3 beta, rhTeck, or rmTeck.
  • Source
    Monoclonal Rat IgG2B Clone # 59106
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse CCL21/6Ckine
    Ser24-Gly133
    Accession # P84444
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse CCL21/6Ckine (Catalog # 457-6C)
  • Immunohistochemistry
    25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Intracellular Staining by Flow Cytometry
    2.5 µg/106 cells
    See below
    • Mouse CCL21/6Ckine Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse CCL21/6Ckine Antibody (Catalog # MAB457)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CCL21/6Ckine Biotinylated Antibody (Catalog # BAF457)
  • ELISA Standard
     
    Recombinant Mouse CCL21/6Ckine Protein (Catalog # 457-6C)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
CCL21/6Ckine in Mouse Thymus. CCL21/6Ckine was detected in perfusion fixed frozen sections of mouse thymus using Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB457) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Intracellular Staining by Flow Cytometry
Detection of CCL21/6Ckine in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse CCL21/6Ckine Monoclonal Antibody (Catalog # MAB457, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).  To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

 

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL21/6Ckine

6Ckine is a novel CC chemokine discovered independently by three groups from the EST database. 6Ckine, also named SLC (secondary lymphoid-tissue chemokine), CCL21 and Exodus-2, shows 21‑33% identity to other CC chemokines. 6Ckine contains the four conserved cysteines characteristic of beta chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6Ckine cDNA encodes a 134 amino acid, highly basic, precursor protein with a 23 aa signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6Ckine cDNA encodes a 133 amino acid protein with a 23 signal peptide that is cleaved to generate the 110 aa mature protein. Human and mouse 6Ckine are highly conserved, exhibiting 86% aa sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6Ckine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6Ckine gene location is within a region of about 100 kb from the MIP-3 beta /ELC gene, another identified novel CC chemokine. Unlike most CC chemokines, 6Ckine is not chemotactic for monocytes. Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells. Recombinant human 6Ckine has been shown to be chemotactic for some human T cell lines, resting PBL, and cultured T cells expanded with PHA and IL-2. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dose-dependent manner. 6Ckine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines.

  • References:
    1. Hedrick, J.A. and A. Zlotnik (1997) J. Immunol. 159:1589.
    2. Hromas, R. et al. (1997) J. Immunol. 159:2554.
    3. Nagira, M. et al. (1997) J. Biol. Chem. 272:19518.
  • Entrez Gene IDs:
    6366 (Human); 18829 (Mouse); 298006 (Rat)
  • Alternate Names:
    6Ckine; 6CkineSmall-inducible cytokine A21; Beta-chemokine exodus-2; CCL21; chemokine (C-C motif) ligand 21; CKb9; exodus-2; member 21; SCYA21; SCYA21MGC34555; secondary lymphoid tissue chemokine; SLC; SLCSecondary lymphoid-tissue chemokine; TCA-4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Coordination between CCR7- and CCR9-mediated chemokine signals in prevascular fetal thymus colonization.
    Authors: Liu C, Saito F, Liu Z, Lei Y, Uehara S, Love P, Lipp M, Kondo S, Manley N, Takahama Y
    Blood, 2006;108(8):2531-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Thymic T cell development and progenitor localization depend on CCR7.
    Authors: Misslitz A, Pabst O, Hintzen G, Ohl L, Kremmer E, Petrie HT, Forster R
    J. Exp. Med., 2004;200(4):481-91.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Herpes simplex virus (HSV) amplicon-mediated codelivery of secondary lymphoid tissue chemokine and CD40L results in augmented antitumor activity.
    Authors: Tolba KA, Bowers WJ, Muller J, Housekneckt V, Giuliano RE, Federoff HJ, Rosenblatt JD
    Cancer Res., 2002;62(22):6545-51.
    Species: Human
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  4. Defective CCR7 expression on dendritic cells contributes to the development of visceral leishmaniasis.
    Authors: Ato M, Stager S, Engwerda CR, Kaye PM
    Nat. Immunol., 2002;3(12):1185-91.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
Cell and Tissue Staining Kits
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Anti-Rat HRP-DAB Cell & Tissue Staining Kit

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Rat IgG2B Isotype Control

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Normal Rat IgG Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
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Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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