Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Western Blot, Flow Cytometry, Immunoprecipitation, Functional Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all CD300f/LMIR3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD300f/LMIR3
Cys16-Gly188
Accession # Q6SJQ7

Specificity

Detects mouse CD300f/LMIR3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) LMIR1, rhLMIR2, rhLMIR3, recombinant mouse LMIR5, and rhLMIR6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse CD300f/LMIR3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: RAW 264.7 mouse monocyte/macrophage cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse CD300f/LMIR3

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD300f/LMIR3

Mouse CD300f, also known as LMIR3, CLM-1, IgSF13, IREM-1 and CMRF35-like molecule 1, is a 55 kDa type I transmembrane (TM) glycoprotein. It is synthesized as a 283 amino acid (aa) precursor that contains a putative 19 aa signal sequence, a 175 aa extracellular region that contains one Ig-like domain, a 17 aa TM segment and a 72 aa cytoplasmic domain that contains an ITIM sequence. There are multiple alleles for this gene that vary considerably in size. Mouse LMIR3 is expressed in monocytic cell lines. Mature mouse LMIR3 extracellular region shares 43% and 72% aa identity with human and rat LMIR3 extracellular region, respectively.

Long Name

Leukocyte Mono Ig-like Receptor 3

Alternate Names

CD300f, CD300LF, CLIM1, CLM1, DIgR2, IGSF13, IREM-1, LMIR3, NKIR, Pigr3

Entrez Gene IDs

146722 (Human); 246746 (Mouse)

Gene Symbol

CD300LF

UniProt

Q6SJQ7

Additional CD300f/LMIR3 Products

Product Documents for Mouse CD300f/LMIR3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CD300f/LMIR3 Antibody

For research use only

Citations for Mouse CD300f/LMIR3 Antibody

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