Mouse CD300f/LMIR3 Antibody

Catalog # Availability Size / Price Qty
AF2788
AF2788-SP
Product Details
Citations (3)
FAQs
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Mouse CD300f/LMIR3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CD300f/LMIR3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) LMIR1, rhLMIR2, rhLMIR3, recombinant mouse LMIR5, and rhLMIR6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CD300f/LMIR3
Cys16-Gly188
Accession # Q6SJQ7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse CD300f/LMIR3
Flow Cytometry
2.5 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD300f/LMIR3

Mouse CD300f, also known as LMIR3, CLM-1, IgSF13, IREM-1 and CMRF35-like molecule 1, is a 55 kDa type I transmembrane (TM) glycoprotein. It is synthesized as a 283 amino acid (aa) precursor that contains a putative 19 aa signal sequence, a 175 aa extracellular region that contains one Ig-like domain, a 17 aa TM segment and a 72 aa cytoplasmic domain that contains an ITIM sequence. There are multiple alleles for this gene that vary considerably in size. Mouse LMIR3 is expressed in monocytic cell lines. Mature mouse LMIR3 extracellular region shares 43% and 72% aa identity with human and rat LMIR3 extracellular region, respectively.

Long Name
Leukocyte Mono Ig-like Receptor 3
Entrez Gene IDs
146722 (Human); 246746 (Mouse)
Alternate Names
CMRF35-like molecule 1; CD300 molecule-like family member f; CD300f; CD300LF; CLIM1; CLM1; CLM-1; DIgR2; IGSF13; IREM1; IREM-1; LMIR3; NKIR; Pigr3

Product Datasheets

Citations for Mouse CD300f/LMIR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Increased hippocampal excitability in miR-324-null mice
    Authors: DJ Hayman, T Modebadze, S Charlton, K Cheung, J Soul, H Lin, Y Hao, CG Miles, D Tsompani, RM Jackson, MD Briggs, KA Piróg, IM Clark, MJ Barter, GJ Clowry, FEN LeBeau, DA Young
    Scientific Reports, 2021;11(1):10452.
    Species: Mouse
    Sample Types: Tissue Lysates
    Applications: Western Blot
  2. Mouse LIMR3/CD300f is a negative regulator of the antimicrobial activity of neutrophils
    Authors: K Ueno, M Urai, K Izawa, Y Otani, N Yanagihara, M Kataoka, S Takatsuka, M Abe, H Hasegawa, K Shimizu, T Kitamura, J Kitaura, Y Miyazaki, Y Kinjo
    Sci Rep, 2018;8(1):17406.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. An activating and inhibitory signal from an inhibitory receptor LMIR3/CLM-1: LMIR3 augments lipopolysaccharide response through association with FcRgamma in mast cells.
    Authors: Izawa K, Kitaura J, Yamanishi Y, Matsuoka T, Kaitani A, Sugiuchi M, Takahashi M, Maehara A, Enomoto Y, Oki T, Takai T, Kitamura T
    J. Immunol., 2009;183(2):925-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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