Mouse Collagen I  alpha 1 Antibody

COL1A1 in Mouse Colon via seqIF™ staining on COMET™ COL1A1 was detected in immersion fixed paraffin-embedded sections of mouse Colon using Rat Anti-Mouse COL1A1, Monoclonal Antibody (Catalog #MAB11700) at 25ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100).. Specific staining was localized to connective tissue. Protocol available in COMET™ Panel Builder.​

Detection of Collagen I  alpha 1 in Mouse Kidney. Collagen I  alpha 1 was detected in perfusion fixed paraffin-embedded sections of mouse kidney using Rat Anti-Mouse Collagen I  alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Collagen I  alpha 1 in Mouse Pancreas. Collagen I  alpha 1 was detected in perfusion fixed paraffin-embedded sections of mouse pancreas using Rat Anti-Mouse Collagen I  alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Detection of Collagen I  alpha 1 in 3T3-L1 cells. Collagen I  alpha 1 was detected in immersion fixed 3T3‑L1 mouse embryonic fibroblast adipose-like cell line (Positive) and absent in RAW 264.7 mouse monocyte/macrophage cell line (Negative) using Rat Anti-Mouse Collagen I  alpha 1 Monoclonal Antibody (Catalog # MAB11700) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Mouse Collagen I  alpha 1 by Western Blot. Western Blot shows lysates of MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/ml of Rat Anti-Mouse Collagen I  alpha 1 Monoclonal Antibody (Catalog # MAB11700) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Collagen I  alpha 1 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Mouse Collagen I  alpha 1 by Simple Western^TM. Simple Western lane view shows lysates of MEF mouse embryonic feeder cells, loaded at 0.1 mg/ml. A specific band was detected for Collagen I  alpha 1 at approximately 226 kDa (as indicated) using 10 µg/ml of Rat Anti-Mouse Collagen I  alpha 1 Monoclonal Antibody (Catalog # MAB11700) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) in Milk-free Antibody Diluent (Catalog # 043-524). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.