CXCL1/GRO alpha/KC/CINC-1 is an ELR+ angiogenic chemokine that interacts with CXCR2/IL-8 RB to attract and activate neutrophils. It is produced at sites of microbial infection by macrophages, monocytes, Kupffer cells, hepatocytes, endothelial and epithelial cells, cardiac and vascular smooth muscle cells, fibroblasts, melanocytes, mast cells, and neurons. CXCL1 function is associated with the development and extent of atherosclerosis, Alzheimer’s disease, and tumor angiogenesis.
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Mouse CXCL1/KC DuoSet ELISA
R&D Systems | Catalog # DY453
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Key Product Details
Assay Type
Solid Phase Sandwich ELISA
Assay Range
15.6-1000 pg/mL
Sample Type
Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet
Reactivity
Mouse
Mouse CXCL1/KC DuoSet ELISA Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
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Product Summary for Mouse CXCL1/KC DuoSet ELISA
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant mouse KC. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
Product Specifications
Assay Format
96-well strip plate (sold separately)
Sample Volume Required
100 µL
Detection Method
Colorimetric ELISA - 450nm (TMB)
Conjugate
Biotin
Specificity
Label
HRP
Scientific Data Images for Mouse CXCL1/KC DuoSet ELISA
Mouse CXCL1 / GRO alpha / KC / CINC-1 ELISA Standard Curve
Kit Contents for Mouse CXCL1/KC DuoSet ELISA
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.
The components listed above may be purchased separately:
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or 0.05% Tween® 20 in PBS, pH 7.2-7.4
Reagent Diluent: (Catalog # DY995), or 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990)
Plate Sealers: ELISA Plate Sealers (Catalog # DY992)
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL1/GRO alpha/KC/CINC-1
Alternate Names
CINC-1, CINC1, GRO alpha, KC, MGSA-alpha
Gene Symbol
CXCL1
Additional CXCL1/GRO alpha/KC/CINC-1 Products
Product Documents for Mouse CXCL1/KC DuoSet ELISA
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse CXCL1/KC DuoSet ELISA
For research use only
Related Research Areas
Citations for Mouse CXCL1/KC DuoSet ELISA
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Customer Reviews for Mouse CXCL1/KC DuoSet ELISA (19)
4.5 out of 5
19 Customer Ratings
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Sample Tested: SerumVerified Customer | Posted 08/29/2023
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Sample Tested: SerumVerified Customer | Posted 05/04/2023
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Sample Tested: SerumVerified Customer | Posted 06/27/2022Stable and robust kit
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Sample Tested: mouse tumor lysatesVerified Customer | Posted 10/20/2021
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Sample Tested: mouse serumVerified Customer | Posted 12/09/2020
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Sample Tested: mouse serumVerified Customer | Posted 06/19/2020The kit worked great. We used it to test the serum level of KC from mice infected by bacterial pathogen. It showed significant induction of KC in infected mice.
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Sample Tested: MH-S murine alveolar macrophages cell lineVerified Customer | Posted 05/01/2020
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Sample Tested: mouse macrophagesVerified Customer | Posted 09/11/2019
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Sample Tested: Mouse Lung BALFVerified Customer | Posted 05/22/2019When protocol is followed as described, standard curve is good.
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Sample Tested: activated mouse CD8 T cellVerified Customer | Posted 12/28/2017
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Sample Tested: Mouse lung BALFVerified Customer | Posted 12/04/2017Result has been published in 10.1371/journal.pone.0058258
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Sample Tested: Cell culture supernatantVerified Customer | Posted 10/31/2017
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Sample Tested: Small intestine tissueVerified Customer | Posted 09/08/2017
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Sample Tested: SerumVerified Customer | Posted 05/09/2017
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Sample Tested: Cell culture supernatantVerified Customer | Posted 10/06/2016
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Sample Tested: BAL fluidVerified Customer | Posted 07/05/2016
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Sample Tested: Blood Serum and Cell culture supernatantVerified Customer | Posted 06/03/2016
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Sample Tested: murine lung homogenateVerified Customer | Posted 04/08/2016easy to use, consistent results
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Sample Tested: Cell culture supernatant and Bone marrow-derived dendritic cellsVerified Customer | Posted 02/12/2016Murine bone marrow derived dendritic cells were propagated in bento(a)pyrene and exposed to heat killed pseudomonas aeruginosa (HKPA) for 24 hours. Levels of KC were assessed in the tissue cultures supernatants by ELISA. n=5, triplicate wells. * p < 0.05
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Protocols
View specific protocols for Mouse CXCL1/KC DuoSet ELISA (DY453):
GENERAL ELISA PROTOCOL
Plate Preparation
- Dilute the Capture Antibody to the working concentration in PBS without carrier protein. Immediately coat a 96-well microplate with 100 μL per well of the diluted Capture Antibody. Seal the plate and incubate overnight at room temperature.
- Aspirate each well and wash with Wash Buffer, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (400 μL) using a squirt bottle, manifold dispenser, or autowasher. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Buffer by aspirating or by inverting the plate and blotting it against clean paper towels.
- Block plates by adding 300 μL Reagent Diluent to each well. Incubate at room temperature for a minimum of 1 hour.
- Repeat the aspiration/wash as in step 2. The plates are now ready for sample addition.
Assay Procedure
- Add 100 μL of sample or standards in Reagent Diluent, or an appropriate diluent, per well. Cover with an adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the Detection Antibody, diluted in Reagent Diluent, to each well. Cover with a new adhesive strip and incubate 2 hours at room temperature.
- Repeat the aspiration/wash as in step 2 of Plate Preparation.
- Add 100 μL of the working dilution of Streptavidin-HRP to each well. Cover the plate and incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Repeat the aspiration/wash as in step 2.
- Add 100 μL of Substrate Solution to each well. Incubate for 20 minutes at room temperature. Avoid placing the plate in direct light.
- Add 50 μL of Stop Solution to each well. Gently tap the plate to ensure thorough mixing.
- Determine the optical density of each well immediately, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. If wavelength correction is not available, subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
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- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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