Mouse CXCL9/MIG Antibody

(17 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CXCL9/MIG in ELISAs and Western blots. In sandwich immunoassays, less than 0.5% cross-reactivity with recombinant human (rh) CXCL9/MIG is observed and less than 0.005% cross-reactivity with recombinant mouse MIP-2, recombinant rat CINC-1, rhGRO alpha, rhGRO beta, and rhGRO gamma is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse CXCL9/MIG
    Accession # P18340
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
    • Mouse CXCL9/MIG Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse CXCL9/MIG Antibody (Catalog # AF-492-NA)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse CXCL9/MIG Biotinylated Antibody (Catalog # BAF492)
  • ELISA Standard
     
    Recombinant Mouse CXCL9/MIG Protein (Catalog # 492-MM)
  • Neutralization
    Measured by its ability to neutralize CXCL9/MIG-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR3. The Neutralization Dose (ND50) is typically 6-20 µg/mL in the presence of 1 µg/mL Recombinant Mouse CXCL9/MIG.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse CXCL9/MIG by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CXCL9/MIG at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chemotaxis Induced by CXCL9/MIG and Neutralization by Mouse CXCL9/MIG Antibody. Recombinant Mouse CXCL9/MIG (Catalog #
492-MM) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL9/MIG (1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL9/MIG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-492-NA). The ND50 is typically 6-20 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCL9/MIG

CXCL9, also known as MIG, is a member of the alpha  subfamily of chemokines that lacks the ELR domain, and was initially identified as a lymphokine-activated gene in mouse macrophages. Human CXCL9 was subsequently cloned using mouse CXCL9 cDNA as a probe. The CXCL9 gene is induced in macrophages and in primary glial cells of the central nervous system specifically in response to IFN-gamma. CXCL9 has been shown to be a chemoattractant for activated T-lymphocytes and TIL but not for neutrophils or monocytes. The mouse CXCL9 cDNA encodes a 126 amino acid residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield a 105 amino acid residue mature protein. CXCL9 has an extended carboxy-terminus containing greater than 50% basic amino acid residues and is larger than most other chemokines. The carboxy-terminal residues of CXCL9 are prone to proteolytic cleavage resulting in size heterogeneity of natural and recombinant CXCL9. CXCL9 with large carboxy-terminal deletions have been shown to have diminished activity in the calcium flux assay. A chemokine receptor (CXCR3) specific for CXCL9 and IP-10 has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

  • Entrez Gene IDs:
    4283 (Human); 17329 (Mouse); 246759 (Rat)
  • Alternate Names:
    chemokine (C-X-C motif) ligand 9; CMK; crg-10; C-X-C motif chemokine 9; CXCL9; Gamma-interferon-induced monokine; Humig; MIG; MIGSmall-inducible cytokine B9; monokine induced by gamma interferon; SCYB9Monokine induced by interferon-gamma
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Herpes simplex virus-1 evasion of CD8+ T cell accumulation contributes to viral encephalitis
    Authors: N Koyanagi, T Imai, K Shindo, A Sato, W Fujii, T Ichinohe, N Takemura, S Kakuta, S Uematsu, H Kiyono, Y Maruzuru, J Arii, A Kato, Y Kawaguchi
    J. Clin. Invest., 2017;0(0):.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  2. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Capture
  3. Salt suppresses IFN? inducible chemokines through the IFN?-JAK1-STAT1 signaling pathway in proximal tubular cells
    Authors: Y Arai, D Takahashi, K Asano, M Tanaka, M Oda, SBH Ko, MSH Ko, S Mandai, N Nomura, T Rai, S Uchida, E Sohara
    Sci Rep, 2017;7(0):46580.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. Optimal CD4 T cell priming after LPS-based adjuvanticity with CD134 costimulation relies on CXCL9 production
    Authors: P Shinde, W Liu, A Ménoret, AD Luster, AT Vella
    J. Leukoc. Biol., 2017;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  5. Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone
    Nat Commun, 2016;7(0):13885.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  6. CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  7. Regulatory T cells prevent inducible BALT formation by dampening neutrophilic inflammation.
    Authors: Foo S, Zhang V, Lalwani A, Lynch J, Zhuang A, Lam C, Foster P, King C, Steptoe R, Mazzone S, Sly P, Phipps S
    J Immunol, 2015;194(9):4567-76.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Tumor cell-activated CARD9 signaling contributes to metastasis-associated macrophage polarization.
    Authors: Yang M, Shao J, Miao Y, Cui W, Qi Y, Han J, Lin X, Du J
    Cell Death Differ, 2014;21(8):1290-302.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  9. Trafficking of high avidity HER-2/neu-specific T cells into HER-2/neu-expressing tumors after depletion of effector/memory-like regulatory T cells.
    Authors: Weiss VL, Lee TH, Song H, Kouo TS, Black CM, Sgouros G, Jaffee EM, Armstrong TD
    PLoS ONE, 2012;7(2):e31962.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  10. A novel combination immunotherapy for cancer by IL-13Ralpha2-targeted DNA vaccine and immunotoxin in murine tumor models.
    Authors: Nakashima H, Terabe M, Berzofsky JA, Husain SR, Puri RK
    J. Immunol., 2011;187(10):4935-46.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  11. Comprehensive assessment of chemokine expression profiles by flow cytometry.
    Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
    J. Clin. Invest., 2010;120(3):907-23.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  12. Monokine induced by interferon-gamma (MIG/CXCL9) is derived from both donor and recipient sources during rejection of class II major histocompatibility complex disparate skin allografts.
    Authors: Auerbach MB, Shimoda N, Amano H, Rosenblum JM, Kish DD, Farber JM, Fairchild RL
    Am. J. Pathol., 2009;174(6):2172-81.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  13. The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
    Authors: Fujita H, Asahina A, Komine M, Tamaki K
    Cell. Immunol., 2007;245(2):70-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  14. Viral infection of the lungs through the eye.
    Authors: Bitko V, Musiyenko A, Barik S
    J. Virol., 2007;81(2):783-90.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  15. L-selectin-negative CCR7- effector and memory CD8+ T cells enter reactive lymph nodes and kill dendritic cells.
    Authors: Guarda G, Hons M, Soriano SF, Huang AY, Polley R, Martin-Fontecha A, Stein JV, Germain RN, Lanzavecchia A, Sallusto F
    Nat. Immunol., 2007;8(7):743-52.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  16. IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice.
    Authors: Di Carlo E, Comes A, Orengo AM, Rosso O, Meazza R, Musiani P, Colombo MP, Ferrini S
    J. Immunol., 2004;172(3):1540-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh/Frozen
  17. Tumor necrosis factor-dependent segmental control of MIG expression by high endothelial venules in inflamed lymph nodes regulates monocyte recruitment.
    Authors: Janatpour MJ, Hudak S, Sathe M, McEvoy LM
    J. Exp. Med., 2001;194(9):1375-84.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Expand to show all 17 Citations
Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

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Goat IgG HRP-conjugated Antibody

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Goat IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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