Mouse CXCR2/IL-8RB Antibody (242216)

R&D Systems | Catalog # MAB2164

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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Neutralization, Flow Cytometry, CyTOF-reported

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay, In vivo assay, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 242216
View all CXCR2/IL-8RB Primary Antibodies »

Product Specifications

Immunogen

C6 rat glioma cell line transfected with mouse CXCR2
Met1-Leu359
Accession # P35343

Specificity

Stains mouse CXCR2 transfected cells but not CXCR1 transfectants.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CXCR2/IL-8RB Antibody (242216)

Detection of CXCR2/IL-8 RB antibody in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP antibody by Flow Cytometry.

Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).
CXCR2/IL-8 RB antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr).

CXCR2/IL‑8 RB in Mouse Spleen.

CXCR2/IL-8 RB was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell sufaces in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Chemotaxis Induced by CXCL2/MIP‑2 and Neutralization by Mouse CXCR2/IL‑8 RB Antibody.

Chemotaxis Induced by CXCL2/MIP‑2 and Neutralization by Mouse CXCR2/IL‑8 RB Antibody.

Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164). The ND50 is typically 15-50 µg/mL.

Applications for Mouse CXCR2/IL-8RB Antibody (242216)

Application
Recommended Usage

CyTOF-reported

Wang, G. et al. (2016) Cancer Discov. 6: 80. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 human embryonic kidney cell line transfected with mouse CXCR2/IL-8 RB and eGFP

Immunohistochemistry

5-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse spleen

Neutralization

Measured by its ability to neutralize CXCL2/GRO beta /MIP‑2/CINC‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR2. The Neutralization Dose (ND50) is typically 15-50 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/GRO beta /MIP‑2/CINC‑3.

Reviewed Applications

Read 1 review rated 5 using MAB2164 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CXCR2/IL-8RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

Long Name

Interleukin 8 Receptor B

Alternate Names

CD182, CDw128b, CMKAR2, CXCR-2, IL-8 RB, IL8RB

Entrez Gene IDs

3579 (Human); 12765 (Mouse); 29385 (Rat)

Gene Symbol

CXCR2

UniProt

P35343

Additional CXCR2/IL-8RB Products

Product Documents for Mouse CXCR2/IL-8RB Antibody (242216)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse CXCR2/IL-8RB Antibody (242216)

For research use only

Citations for Mouse CXCR2/IL-8RB Antibody (242216)

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  • Mouse CXCR2/IL-8RB Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: fibroblasts
    Species: Mouse
    Verified Customer | Posted 08/18/2021
    Mouse CXCR2/IL-8RB Antibody (242216) MAB2164

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Protocols

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