Mouse CXCR2/IL-8 RB Antibody

(25 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Stains mouse CXCR2 transfected cells but not CXCR1 transfectants.
  • Source
    Monoclonal Rat IgG2A Clone # 242216
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    C6 rat glioma cell line transfected with mouse CXCR2
    Met1-Leu359
    Accession # P35343
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-reported
    Wang, G. et al. (2016) Cancer Discov. 6: 80. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Neutralization
    Measured by its ability to neutralize CXCL2/GRO beta /MIP‑2/CINC‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR2. The Neutralization Dose (ND50) is typically 15-50 µg/mL in the presence of 2 ng/mL Recombinant Mouse CXCL2/GRO beta /MIP‑2/CINC‑3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CXCL2/MIP‑2 and Neutralization by Mouse CXCR2/IL‑8 RB Antibody. Recombinant Mouse CXCL2/MIP‑2 (Catalog #
452-M2) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP‑2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB2164). The ND50 is typically 15-50 µg/mL.
Detection of CXCR2/IL‑8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse
CXCR1/IL-8 RA  and eGFP was stained with Rat Anti-Mouse CXCR2/IL‑8 RB Monoclonal Antibody (Catalog # MAB2164) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CXCR2/IL-8 RB

CXCR2, also known as IL-8 RB, is a G protein-coupled chemokine receptor expressed on neutrophils. It binds IL-8, GRO alpha, GRO beta, GRO gamma, NAP-2, and ENA-78.

  • Long Name:
    Interleukin 8 Receptor B
  • Entrez Gene IDs:
    3579 (Human); 12765 (Mouse); 29385 (Rat)
  • Alternate Names:
    beta; CD182 antigen; CD182; CDw128b; chemokine (C-X-C motif) receptor 2; chemokine (CXC) receptor 2; C-X-C chemokine receptor type 2; CXCR2 gene for IL8 receptor type B; CXCR2; CXC-R2; CXCR-2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8 receptor type 2; IL-8R B; IL8R2; IL8RA; IL8RB; IL-8RB; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin-8 receptor type B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis
    Authors: K Watanabe, CA Gilchrist, MJ Uddin, SL Burgess, MM Abhyankar, SN Moonah, Z Noor, JR Donowitz, BN Schneider, T Arju, E Ahmed, M Kabir, M Alam, R Haque, P Pramoonjag, B Mehrad, WA Petri
    PLoS Pathog., 2017;13(8):e1006513.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  2. The mTOR signal regulates myeloid-derived suppressor cells differentiation and immunosuppressive function in acute kidney injury
    Authors: C Zhang, S Wang, J Li, W Zhang, L Zheng, C Yang, T Zhu, R Rong
    Cell Death Dis, 2017;8(3):e2695.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.
    Authors: Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce J, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
    J Biol Chem, 2015;290(4):2053-68.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. The calcineurin-NFAT axis controls allograft immunity in myeloid-derived suppressor cells through reprogramming T cell differentiation.
    Authors: Wang X, Bi Y, Xue L, Liao J, Chen X, Lu Y, Zhang Z, Wang J, Liu H, Yang H, Liu G
    Mol Cell Biol, 2015;35(3):598-609.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  5. Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.
    Authors: Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges M, Benencia F
    Exp Cell Res, 2014;323(1):7-27.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
  6. Platelets guide the formation of early metastatic niches.
    Authors: Labelle, Myriam, Begum, Shahinoo, Hynes, Richard
    Proc Natl Acad Sci U S A, 2014;111(30):E3053-61.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  7. mTOR limits the recruitment of CD11b+Gr1+Ly6Chigh myeloid-derived suppressor cells in protecting against murine immunological hepatic injury.
    Authors: Zhang Y, Bi Y, Yang H, Chen X, Liu H, Lu Y, Zhang Z, Liao J, Yang S, Chu Y, Yang R, Liu G
    J Leukoc Biol, 2014;95(6):961-70.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  8. Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2.
    Authors: Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner R, Moschen A, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf A, Wolf D, Eller P, Ritsch A, Weiss G
    EMBO Mol Med, 2014;6(6):810-20.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  9. Phosphatase Wip1 negatively regulates neutrophil migration and inflammation.
    Authors: Sun B, Hu X, Liu G, Ma B, Xu Y, Yang T, Shi J, Yang F, Li H, Zhang L, Zhao Y
    J Immunol, 2014;192(3):1184-95.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  10. CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-beta.
    Authors: Jablonska J, Wu C, Andzinski L, Leschner S, Weiss S
    Int J Cancer, 2014;134(6):1346-58.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  11. IL-17A produced by neutrophils protects against pneumonic plague through orchestrating IFN-gamma-activated macrophage programming.
    Authors: Bi Y, Zhou J, Yang H, Wang X, Zhang X, Wang Q, Wu X, Han Y, Song Y, Tan Y, Du Z, Yang H, Zhou D, Cui Y, Zhou L, Yan Y, Zhang P, Guo Z, Wang X, Liu G, Yang R
    J Immunol, 2014;192(2):704-13.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  12. G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.
    Authors: Raghuwanshi S, Smith N, Rivers E, Thomas A, Sutton N, Hu Y, Mukhopadhyay S, Chen X, Leung T, Richardson R
    J Immunol, 2013;190(10):5329-36.
    Species: Mouse
    Sample Type: In Vivo
    Application: Neut
  13. Inefficient antimicrobial functions of innate phagocytes render infant mice more susceptible to bacterial infection.
    Authors: Zhang Q, Coveney A, Yu S, Liu J, Li Y, Blankson S, Redmond H, Wang J, Wang J
    Eur J Immunol, 2013;43(5):1322-32.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  14. CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis.
    Authors: Roy M, Richard JF, Dumas A, Vallieres L
    J Neuroinflammation, 2012;9(0):18.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  15. TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines.
    Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG
    J. Lipid Res., 2011;52(7):1307-18.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  16. Defective regulation of CXCR2 facilitates neutrophil release from bone marrow causing spontaneous inflammation in severely NF-kappa B-deficient mice.
    Authors: von Vietinghoff S, Asagiri M, Azar D
    J. Immunol., 2010;185(1):670-8.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  17. Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice.
    Authors: Traylor ZP, Yu EN, Davis IC
    Am. J. Physiol. Lung Cell Mol. Physiol., 2010;298(3):L437-45.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  18. Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.
    Authors: Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(14):5954-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  19. Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1.
    Authors: Omari KM, Lutz SE, Santambrogio L, Lira SA, Raine CS
    Am. J. Pathol., 2009;174(1):164-76.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  20. An age-associated increase in pulmonary inflammation after burn injury is abrogated by CXCR2 inhibition.
    Authors: Nomellini V, Faunce DE, Gomez CR, Kovacs EJ
    J. Leukoc. Biol., 2008;83(6):1493-501.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  21. HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling.
    Authors: Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM
    Blood, 2008;111(7):3343-54.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  22. Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice.
    Authors: Sawanobori Y, Ueha S, Kurachi M, Shimaoka T, Talmadge JE, Abe J, Shono Y, Kitabatake M, Kakimi K, Mukaida N, Matsushima K
    Blood, 2008;111(12):5457-66.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  23. Anti-type II collagen antibody accelerates arthritis via CXCR2-expressing cells in IL-1 receptor antagonist-deficient mice.
    Authors: Kagari T, Tanaka D, Doi H, Iwakura Y, Shimozato T
    Eur. J. Immunol., 2007;37(10):2753-63.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  24. An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals.
    Authors: Yu JJ, Ruddy MJ, Wong GC, Sfintescu C, Baker PJ, Smith JB, Evans RT, Gaffen SL
    Blood, 2007;109(9):3794-802.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  25. Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice.
    Authors: Mohsenin A, Mi T, Xia Y, Kellems RE, Chen JF, Blackburn MR
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(3):L753-61.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
Expand to show all 25 Citations
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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