Mouse EDAR Antibody

R&D Systems | Catalog # AF745

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Blockade of Receptor-ligand Interaction

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EDAR Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EDAR
Glu27-Ala187
Accession # Q9R187

Specificity

Detects mouse EDAR in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse EDAR Antibody

Detection of EDAR by Immunohistochemistry

Detection of EDAR by Immunohistochemistry

EDAR and LEF1 expression in Meis2 cKO mice.(A) Three examples of 10 μm FFPE sections stained with EDAR antibody showing affected expression of EDAR in Meis2 cKO mice. Arrows indicate EDAR-positive sites which are observed less frequently in the mutant snout. Scale bar: 500 μm. (B) Two representative images of normally developed escaper whisker follicles (WFs) in the mutants by LEF1 staining. LEF1 expression is similar in just forming placodes (top) and invaginated (bottom). Additionally, LEF1 expression shows a decline in the DC regions compared to peri-DC. Scale bar: 30 μm. (C) Three representative micrographs of LEF1-stained 10 μm FFPE snout sections showing normal expression of LEF1 in the dermis but the decreased number of LEF1+ placodes in the epithelium of the mutant snout. Scale bar: 500 μm. (D) Quantification of Lef1+ placode count and Lef1 fluorescence intensities in placodes, DCs, or interfollicular upper dermis. Data are presented as mean ± sem, ***p=0.0002. (E) In situ hybridization HCR-FISH using probes for Axin2 and Wnt10b in control snout at embryonic day 12.5 (E12.5) showing placodal expression of Wnt10b (green) and Axin2 (arrows, red) as well as widespread expression of Axin2 in the dermis. Scale bar: 60 μm. Image collected and cropped by CiteAb from the following open publication (https://elifesciences.org/articles/100854), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse EDAR Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 1 μg/mL of biotinylated Recombinant Mouse EDA to immobilized Recombinant Mouse EDAR Fc Chimera (Catalog # 745-ED) coated at 1 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse EDAR Fc Chimera (Catalog # 745-ED)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: EDAR

EDAR is a type I transmembrane protein which is a member of the TNF Receptor Superfamily (TNFRSF). The extracellular domain contains 14 cysteine residues, six of which approximate the TNFRSF cysteine-rich region, the cytoplasmic domain contains a region with homology to the death domains found in other TNFRSF members. Mouse EDAR is a 488 amino acid (aa) protein with a predicted 30 aa signal, a 159 aa extracellular domain, a 22 aa transmembrane domain, and a 277 aa cytoplasmic domain. The human and mouse EDAR homologs share 91% identity. Within the TNFRSF, EDAR shares the highest homologies with XEDAR and TNFRSF19/TROY. EDA-A1 is the EDAR ligand. EDA and EDAR have been associated with hypohidrotic ectodermal dysplasia (HED). HED is characterized by abnormalities in hair, teeth and eccrine sweat gland morphogenesis. HED was initially found to associate with two gene loci, tabby and downless. Tabby was later identified as the gene for EDA and downless as the autosomal EDAR gene. EDA has two splice variants, EDA-A1 and EDA-A2 which differ by only two amino acids. Despite this minor difference, the EDA isoforms display strong receptor specificity. EDA-A1 only binds to EDAR, whereas EDA-A2 binds to XEDAR, an X-linked TNFRSF member with high homology to EDAR. Mutations in EDA, EDAR and XEDAR have been associated with HED.

References

  1. Headon, D.J. and Overbeek, P.A. (1999) Nat. Genet. 22:370.
  2. Kumar, A. et al. (2000) J. Biol. Chem. 276:2668.
  3. Monreal, A.W. et al. (1999) Nat. Genet. 22:366.
  4. Schneider, P. et al. (2001) J.  Biol. Chem. 276:18819.
  5. Srivastava, A.K. et al. (1997) Proc. Natl. Acad. Sci. USA 94:13069.
  6. Yan, M. et al. (2000) Science 209:523.

Long Name

Ectodysplasin Receptor

Alternate Names

Anhidrotic ectodysplasin receptor 1, DLED3, Ectodermal dysplasia receptor, ectodysplasin 1, anhidrotic receptor, ectodysplasin A receptor, Ectodysplasin-A receptor, ED1R, ED5, EDA1R, EDA3EDA-A1 receptor, EDA-A1R, Edar, FLJ94390, HRM1, mouse, homolog of, tumor necrosis factor receptor superfamily member EDAR

Entrez Gene IDs

10913 (Human); 13608 (Mouse); 102120238 (Cynomolgus Monkey)

Gene Symbol

EDAR

UniProt

Q9R187

Additional EDAR Products

Product Documents for Mouse EDAR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse EDAR Antibody

For research use only

Citations for Mouse EDAR Antibody

Customer Reviews for Mouse EDAR Antibody

There are currently no reviews for this product. Be the first to review Mouse EDAR Antibody and earn rewards!

Have you used Mouse EDAR Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies