Epidermal Growth Factor (EGF) is the prototypic member of a family of growth factors that have EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 262930
Loading...
Product Specifications
Immunogen
E. coli-derived recombinant mouse EGF
Asn977-Arg1029
Accession # P01132
Asn977-Arg1029
Accession # P01132
Specificity
Detects mouse EGF in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) Amphiregulin, rmBetacellulin, rhEGF, rmEpigen, rmEpiregulin, rhHB-EGF, rhNRG-1 alpha, rhNRG1-beta 1, or rhTGF-alpha.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Applications for Mouse EGF Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Mouse EGF (Catalog # 2028-EG)
Sample: Recombinant Mouse EGF (Catalog # 2028-EG)
Mouse EGF Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Loading...
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: EGF
Long Name
Epidermal Growth Factor
Alternate Names
HOMG4, URG, Urogastrone
Gene Symbol
EGF
UniProt
Additional EGF Products
Product Documents for Mouse EGF Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse EGF Antibody
For research use only
Related Research Areas
Citations for Mouse EGF Antibody
Powered by Bioz
Customer Reviews for Mouse EGF Antibody
There are currently no reviews for this product. Be the first to review Mouse EGF Antibody and earn rewards!
Have you used Mouse EGF Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
