Mouse EGF Antibody

Catalog #: MAB2028 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB2028-SP
MAB2028-500
MAB2028-100

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse EGF Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse EGF in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) Amphiregulin, rmBetacellulin, rhEGF, rmEpigen, rmEpiregulin, rhHB-EGF, rhNRG-1 alpha, rhNRG1-beta 1, or rhTGF-alpha.

Source

Monoclonal Rat IgG_2A Clone # 262930

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse EGF
Asn977-Arg1029
Accession # P01132

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Mouse EGF (Catalog # 2028-EG)

Mouse EGF Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Mouse EGF Biotinylated Antibody (Catalog # BAF2028)

Standard: Recombinant Mouse EGF Protein, CF (Catalog # 2028-EG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EGF

Epidermal Growth Factor (EGF) is the prototypic member of a family of growth factors that have EGF like domains and activate members of the EGF receptor family. Proteolytic cleavage of a membrane-bound precursor releases mature soluble EGF which interacts with the EGF R to promote proliferation and differentiation of mesenchymal and epithelial cells.

Long Name

Epidermal Growth Factor

Entrez Gene IDs

1950 (Human); 13645 (Mouse); 25313 (Rat)

Alternate Names

beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; hEGF; HOMG4; pro-epidermal growth factor; URG; Urogastrone