Detection of Mouse EMMPRIN/CD147 by Western Blot. Western blot shows lysates of mouse brain tissue and mouse heart tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for EMMPRIN/CD147 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EMMPRIN/CD147 in Mouse Testis. EMMPRIN/CD147 was detected in perfusion fixed frozen sections of mouse testis using Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721) at a 1:250 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to spermatozoa. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.
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