Mouse EMMPRIN/CD147 Antibody Summary
Accession # P18572
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse EMMPRIN/CD147 by Western Blot. Western blot shows lysates of mouse brain tissue and mouse heart tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for EMMPRIN/CD147 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
EMMPRIN/CD147 in Mouse Testis. EMMPRIN/CD147 was detected in perfusion fixed frozen sections of mouse testis using Rabbit Anti-Mouse EMMPRIN/CD147 Monoclonal Antibody (Catalog # MAB7721) at a 1:250 dilution overnight at 4 °C. Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) and counterstained with hematoxylin (blue). Specific staining was localized to spermatozoa. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.
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Cell and Tissue Staining Kits
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