Mouse EphA6 Antibody

R&D Systems | Catalog # AF607

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EphA6 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EphA6
Ser28-Gln546
Accession # Q62413

Specificity

Detects mouse EphA6 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20‑30% cross‑reactivity with recombinant human (rh) EphA6, and less than 5% cross-reactivity with recombinant mouse (rm) EphA3, rmEphA4, rmEphA7, rmEphA8, and rhEphrinA5 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse EphA6 Antibody

Detection of EphA6 by Immunocytochemistry/ Immunofluorescence

Detection of EphA6 by Immunocytochemistry/ Immunofluorescence

Immune cells of patients with RR-MS increased EphA-receptor phosphorylation in a co-culture bioassay. HEK-293T cells were unstimulated or stimulated with recombinant proteins or co-cultured with immune cells of HC or RR-MS patients and immunostained to phosphorylated Eph A2+A3+A4-receptors (p-Eph-r) and DAPI nuclear staining. (A) Representative confocal microscopy images of HEK-293T cells unstimulated or stimulated with 0.5 µg/mL of recombinant human Fc-IgG negative control (hrFc IgG), 0.5 µg/mL human recombinant ephrin-A2 (hrEphrin-A2) protein, and pre-incubated with or without 20 µg/mL anti-ephrin-A2 blocking antibody against ephrin-A2 (anti-ephrin-A2). Magnification ×20; scale = 50 µm. (B) Average of the Eph-receptor phosphorylation analysis shown in A. p < 0.05, * p value of hrEphrin-A2 vs. unstimulated (US), ** p value of hrEphrin-A2 vs. hrFc IgG, *** p value of hrEphrin-A2 vs. hrEphrin-A2+ anti-ephrin-A2. (C) Representative confocal microscope images of immunofluorescence staining for p-Eph-r of 293T-HEK cells co-cultured with immune cells of HC or RR-MS patients −/+ anti-ephrin-A2 antibody. Magnification ×20; scale = 50 µm. (D) Average of the Eph-receptor phosphorylation analysis shown in (C). * p value without vs. with anti-ephrin-A2, ** p value of MS vs. US, *** p value of MS vs. HC. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33671716), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EphA6 by Immunocytochemistry/ Immunofluorescence

Detection of EphA6 by Immunocytochemistry/ Immunofluorescence

Immune cells of patients with RR-MS increased EphA-receptor phosphorylation in a co-culture bioassay. HEK-293T cells were unstimulated or stimulated with recombinant proteins or co-cultured with immune cells of HC or RR-MS patients and immunostained to phosphorylated Eph A2+A3+A4-receptors (p-Eph-r) and DAPI nuclear staining. (A) Representative confocal microscopy images of HEK-293T cells unstimulated or stimulated with 0.5 µg/mL of recombinant human Fc-IgG negative control (hrFc IgG), 0.5 µg/mL human recombinant ephrin-A2 (hrEphrin-A2) protein, and pre-incubated with or without 20 µg/mL anti-ephrin-A2 blocking antibody against ephrin-A2 (anti-ephrin-A2). Magnification ×20; scale = 50 µm. (B) Average of the Eph-receptor phosphorylation analysis shown in A. p < 0.05, * p value of hrEphrin-A2 vs. unstimulated (US), ** p value of hrEphrin-A2 vs. hrFc IgG, *** p value of hrEphrin-A2 vs. hrEphrin-A2+ anti-ephrin-A2. (C) Representative confocal microscope images of immunofluorescence staining for p-Eph-r of 293T-HEK cells co-cultured with immune cells of HC or RR-MS patients −/+ anti-ephrin-A2 antibody. Magnification ×20; scale = 50 µm. (D) Average of the Eph-receptor phosphorylation analysis shown in (C). * p value without vs. with anti-ephrin-A2, ** p value of MS vs. US, *** p value of MS vs. HC. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33671716), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse EphA6 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse EphA6 Fc Chimera (Catalog # 607-A6)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphA6

EphA6, also known as Ehk2 and Hek12 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA6 has been shown to bind ephrin-A2, ephrin-A1, ephrin-A3, ephrin-A4, and ephrin-A5 (2, 3). The extracellular domains of rat and mouse EphA6 share 99% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.

Long Name

Eph Receptor A6

Alternate Names

Ehk2, Hek12

Entrez Gene IDs

285220 (Human); 13840 (Mouse)

Gene Symbol

EPHA6

UniProt

Q62413

Additional EphA6 Products

Product Documents for Mouse EphA6 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse EphA6 Antibody

For research use only

Citations for Mouse EphA6 Antibody

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