Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all EphA7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EphA7 isoform 1
Ala30-Pro554 (Gly540-Glu544 del)
Accession # Q61772

Specificity

Detects mouse EphA7 in direct ELISAs and Western blots. In direct ELISAs, approximately 2% cross‑reactivity with recombinant mouse (rm) EphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, and rmEphA8 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse EphA7 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse EphA7 Fc Chimera (Catalog # 608-A7)

Reviewed Applications

Read 2 reviews rated 5 using AF608 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EphA7

EphA7, also known as Mdk1, Hek11, Ehk3, Ebk, and Cek11 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA7 has been shown to bind ephrin-A2, ephrin-A3, ephrin-A1, ephrin-A4, and ephrin-A5 (2, 3). The extracellular domains of human and mouse EphA7 share greater than 97% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.

Long Name

Eph Receptor A7

Alternate Names

Cek11, Ebk, Ehk3, Hek11, Mdk1

Entrez Gene IDs

2045 (Human); 13841 (Mouse)

Gene Symbol

EPHA7

UniProt

Q61772

Additional EphA7 Products

Product Documents for Mouse EphA7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse EphA7 Antibody

For research use only

Citations for Mouse EphA7 Antibody

Customer Reviews for Mouse EphA7 Antibody (2)

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2 Customer Ratings
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  • Mouse EphA7 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Macrophages
    Species: Mouse
    Verified Customer | Posted 07/14/2021
    Mouse EphA7 Antibody AF608
  • Mouse EphA7 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Tramp-C1 mouse prostate cancer cell line
    Species: Mouse
    Verified Customer | Posted 04/20/2021
    Mouse EphA7 Antibody AF608

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