Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Neutralization

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Ephrin-A1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-A1
Asp19-Ser182
Accession # P52793

Specificity

Detects mouse Ephrin-A1 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Ephrin-A1 is observed and less than 1% cross-reactivity with recombinant mouse (rm) Ephrin-B1, rmEphrin-A5, rmEphrin-A4, rmEphrin-A2, and rmEphrin-B1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Ephrin‑A1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Ephrin-A1 Fc Chimera (Catalog # 602-A1)

Reviewed Applications

Read 1 review rated 4 using AF702 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-A1

Ephrin-A1, also known as B61, LERK-1, and EFL-1, (1) is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A1 has been shown to bind EphA1, EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A1 share 85% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/Ephrin families also appear to play a role in angiogenesis (3).

References

  1. Unified nomenclature for Eph family receptors and their ligands, the ephrins. Eph Nomenclature Committee [letter]. (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.

Alternate Names

B61, EFL-1, EFNA1, EphrinA1, LERK1

Entrez Gene IDs

1942 (Human); 13636 (Mouse)

Gene Symbol

EFNA1

UniProt

P52793

Additional Ephrin-A1 Products

Product Documents for Mouse Ephrin‑A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Ephrin‑A1 Antibody

For research use only

Related Research Areas

Ephrin Ligands and Eph Receptors

Citations for Mouse Ephrin‑A1 Antibody

Customer Reviews for Mouse Ephrin‑A1 Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 24103748
    Species: Mouse
    Verified Customer | Posted 01/09/2015

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