Mouse GDF-15 DuoSet ELISA
Mouse GDF-15 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
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Product Features
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
Kit Content
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Reagent Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Scientific Data
Product Datasheets
Preparation and Storage
Background: GDF-15
Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
Citations for Mouse GDF-15 DuoSet ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 5
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Combined genetic deletion of GDF15 and FGF21 has modest effects on body weight, hepatic steatosis and insulin resistance in high fat fed mice
Authors: S Patel, A Haider, A Alvarez-Gu, G Bidault, JS El-Sayed M, E Guiu-Jurad, JA Tadross, J Warner, J Harrison, S Virtue, F Scurria, I Zvetkova, M Blüher, KS Small, S O'Rahilly, DB Savage
Molecular Metabolism, 2022;65(0):101589.
Species: Mouse
Sample Types: Plasma
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The GDF15-GFRAL axis mediates chemotherapy-induced fatigue in mice
Authors: B Chelette, CL Chidomere, R Dantzer
Brain, Behavior, and Immunity, 2022;108(0):45-54.
Species: Mouse
Sample Types: Plasma
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Aldehyde-driven transcriptional stress triggers an anorexic DNA damage response
Authors: L Mulderrig, JI Garaycoech, ZK Tuong, CL Millington, FA Dingler, JR Ferdinand, L Gaul, JA Tadross, MJ Arends, S O'Rahilly, GP Crossan, MR Clatworthy, KJ Patel
Nature, 2021;600(7887):158-163.
Species: Mouse
Sample Types: Serum
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Development of insulin resistance preceded major changes in iron homeostasis in mice fed a high-fat diet
Authors: J Varghese, JV James, R Anand, M Narayanasa, G Rebekah, B Ramakrishn, AJ Nellickal, M Jacob
J. Nutr. Biochem., 2020;84(0):108441.
Species: Mouse
Sample Types: Serum
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Growth differentiation factor 15 ameliorates nonalcoholic steatohepatitis and related metabolic disorders in mice
Authors: KH Kim, SH Kim, DH Han, YS Jo, YH Lee, MS Lee
Sci Rep, 2018;8(1):6789.
Species: Mouse
Sample Types: Serum
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