Mouse GFR alpha -like Antibody

Catalog # Availability Size / Price Qty
AF5728
AF5728-SP

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Detection of Mouse GFR alpha -like by Western Blot.
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Mouse GFR alpha -like Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GFR alpha -like in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse GFR alpha -like isoform A
Gln20-Gly349
Accession # Q6SJE0
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Mouse GFR alpha -like by Western Blot. Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, mouse brain and mouse brain stem. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse GFRa-like Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5728) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for GFRa-like at approximately 25 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry View Larger

GFR alpha ‑like in Mouse Spinal Cord. GFR alpha ‑like was detected in immersion fixed paraffin-embedded sections of mouse spinal cord using Sheep Anti-Mouse GFR alpha ‑like Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5728) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with DAPI (blue). Specific staining was localized to dorsal and ventral horns. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry GFRa-like antibody in Mouse Brain by Immunohistochemistry (IHC-Fr). View Larger

GFR alpha -like in Mouse Brain. GFRa-like was detected in immersion fixed frozen sections of mouse brain using Sheep Anti-Mouse GFRa-like Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5728) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with Fluoro Nissl Green (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-like

GFR alpha -like (GDNF receptor-alpha-like) is a predicted 26 and 44 kDa glycoprotein (1) and distant member of the GDNFR family of proteins. It is expressed on both fetal and adult neurons of the CNS, and would appear to function as an anti-apoptotic molecule during neuronal stress. Mature mouse GFR alpha -like is a 374 amino acid (aa) type I transmembrane protein. It contains a 330 aa extracellular region that shows a Gas1 domain (aa 131‑210), and a 23 aa cytoplasmic tail. There is one splice variant that is likely soluble and shows a four aa substitution for aa 235‑393. Over aa 20‑349, mouse GFR alpha -like shares 72% aa identity with human GFR alpha -like.

References
  1. Li, Z. et al. (2005) J. Neurochem. 95:361.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha-like
Entrez Gene IDs
389400 (Human); 404194 (Mouse); 501023 (Rat)
Alternate Names
GFR alpha like; GFR alpha-like; GFRAL; GRAL

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