Mouse GFR alpha ‑like Antibody

Detection of GFR alpha -like by Immunocytochemistry/ Immunofluorescence

Neuronal activation in CPT-treated mice.DIO mice (weighing 40 to 42 g) received vehicle or CPT (1 mg kg−1); 60 min later, brains were harvested, and tissue sections were subjected to immunofluorescence staining with anti-c-Fos (red) and anti-GFRAL (green). (A) Representative immunofluorescence image of the AP/NTS in CPT- or vehicle-treated DIO mice showing the distribution of GFRAL neurons and the colocalization with the marker of neuronal activation c-Fos. (B) Quantification of CPT-induced c-Fos expression in the AP/NTS (n = 6 per group). Data are presented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001. The underlying data for this figure can be found in S1 Data. AP, area postrema; CPT, Camptothecin; DIO, diet-induced obese; GFRAL, GDNF receptor alpha-like; NTS, nucleus tractus solitarius. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35202387), licensed under a CC-BY license. Not internally tested by R&D Systems.