Mouse Glycoprotein V/CD42d Antibody

Catalog #: AF6990 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6990
AF6990-SP

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Detection of Mouse Glycoprotein V/CD42d by Western Blot.

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Mouse Glycoprotein V/CD42d Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Glycoprotein V/CD42d in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Glycoprotein V/CD42d
Gln17-Gly522
Accession # NP_032174

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Glycoprotein V/CD42d by Western Blot. Western blot shows lysates of mouse platelets. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Glycoprotein V/ CD42d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6990) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Glycoprotein V/CD42d at approximately 70-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Glycoprotein V/CD42d in Mouse Platelets. Glycoprotein V/CD42d was detected in immersion fixed mouse platelets using Sheep Anti-Mouse Glycoprotein V/CD42d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6990) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glycoprotein V/CD42d

GP-V (platelet glycoprotein V; also CD42d) is a 78-88 kDa member of the leucine-rich (LR) repeat family of proteins. It is expressed on the surface of platelets where it noncovalently interacts with three additional proteins to form a heteromeric complex that serves as a receptor for both thrombin and von Willebrand factor. In addition, GP-V is normally a receptor for collagen. Following receptor complex activation due to thrombin cleavage of GP-V, the collagen-binding activity of GP-V is lost, limiting thrombus growth at sites of clot formation. Mature mouse GP-V is a 551 amino acid (aa) type I transmembrane glycoprotein. In its 506 aa extracellular domain (aa 17-522), GP-V contains 16 LR repeats (aa 17-474), and terminates with a short 24 aa cytoplasmic tail. Based on human, mouse GP-V might be naturally cleaved by thrombin and ADAM17, generating soluble fragments that range from 70-82 kDa in size. Over aa 17-522, mouse GP-V shares 83% and 70% aa sequence identity with rat and human GP-V, respectively.

Entrez Gene IDs

2814 (Human); 14729 (Mouse); 25259 (Rat)

Alternate Names

platelet glycoprotein V; CD42d; glycoprotein V (platelet); Glycoprotein V; GP5; GPV