Mouse Granzyme B Biotinylated Antibody

Name: Mouse Granzyme B Biotinylated Antibody
Brand: R&D Systems
SKU: BAF1865
Price: 579 USD
Availability: InStock

R&D Systems | Catalog # BAF1865

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Citations (10)

Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, ELISA Detection (Matched Antibody Pair), Immunocytochemistry

Cited:

Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry, ELISA Development (Detection), In vivo assay

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all Granzyme B Primary Antibodies »

Product Specifications

Specificity

Detects mouse Granzyme B in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) Granzyme A, rhGranzyme B, recombinant mouse (rm) Granzyme D, rmGranzyme G, and rhGranzyme H is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse Granzyme B Biotinylated Antibody

Granzyme B in Mouse Spleen.

Granzyme B was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Poly-clonal Antibody (Catalog # BAF1865) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Granzyme B in Mouse Splenocytes.

Granzyme B was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse Granzyme B Biotinylated Antibody

Application

Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse spleen

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Granzyme B (Catalog # 1865-SE)

Mouse Granzyme B Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)

Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:

Capture Reagent: Mouse Granzyme B Antibody (Catalog # AF1865)
Standard: Recombinant Mouse Granzyme B Protein, CF (Catalog # 1865-SE)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.

References

Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
Lobe, C.G. et al. (1986) Science 232:858.
Brunet, J.F. et al. (1986) Nature 322:268.