Mouse Granzyme B Biotinylated Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Granzyme B in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) Granzyme A, rhGranzyme B, recombinant mouse (rm) Granzyme D, rmGranzyme G, and rhGranzyme H is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Granzyme B
    Lys17-Ser247
    Accession # P04187
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Granzyme B (Catalog # 1865-SE)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed mouse peripheral blood mononuclear cells treated with PMA and calcium ionomycin
    • Mouse Granzyme B Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    0.2-0.8 µg/mL 
    Mouse Granzyme B Antibody (Catalog # AF1865)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Granzyme B Biotinylated Antibody (Catalog # BAF1865)
  • ELISA Standard
     
    Recombinant Mouse Granzyme B Protein, CF (Catalog # 1865-SE)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Granzyme B in Mouse Spleen. Granzyme B was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Poly­clonal Antibody (Catalog # BAF1865) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.

  • References:
    1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
    2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
    3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
    4. Lobe, C.G. et al. (1986) Science 232:858.
    5. Brunet, J.F. et al. (1986) Nature 322:268.
  • Entrez Gene IDs:
    3002 (Human); 14939 (Mouse)
  • Alternate Names:
    CSPB; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Mast cells decrease efficacy of anti-angiogenic therapy by secreting matrix-degrading granzyme B
    Authors: M Wroblewski, R Bauer, M Cubas Córd, F Udonta, I Ben-Batall, K Legler, C Hauser, J Egberts, M Janning, J Velthaus, C Schulze, K Pantel, C Bokemeyer, S Loges
    Nat Commun, 2017;8(1):269.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  2. Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice.
    Authors: Whitehead M, Ohlschlager P, Almajhdi F, Alloza L, Marzabal P, Meyers A, Hitzeroth I, Rybicki E
    BMC Cancer, 2014;14(0):367.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ELISA detection
  3. Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B.
    Authors: Carnevalli L, Scognamiglio R, Cabezas-Wallscheid N, Rahmig S, Laurenti E, Masuda K, Jockel L, Kuck A, Sujer S, Polykratis A, Erlacher M, Pasparakis M, Essers M, Trumpp A
    J Exp Med, 2014;211(5):769-79.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  4. Hematopoietic stem cell function and survival depend on c-Myc and N-Myc activity.
    Authors: Laurenti E, Varnum-Finney B, Wilson A, Ferrero I, Blanco-Bose WE, Ehninger A, Knoepfler PS, Cheng PF, MacDonald HR, Eisenman RN, Bernstein ID, Trumpp A
    Cell Stem Cell, 2008;3(6):611-24.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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Isotype Controls
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