Mouse Granzyme B Biotinylated Antibody Summary
Applications
Mouse Granzyme B Sandwich Immunoassay
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Granzyme B in Mouse Splenocytes. Granzyme B was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Granzyme B in Mouse Spleen. Granzyme B was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Poly-clonal Antibody (Catalog # BAF1865) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
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Preparation and Storage
Background: Granzyme B
Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.
- Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
- Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
- Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
- Lobe, C.G. et al. (1986) Science 232:858.
- Brunet, J.F. et al. (1986) Nature 322:268.
Product Datasheets
Citations for Mouse Granzyme B Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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NK cell-derived extracellular granzyme B drives epithelial ulceration during HSV-2 genital infection
Authors: YS Lim, AG Lee, X Jiang, JM Scott, A Cofie, S Kumar, D Kennedy, DJ Granville, H Shin
Cell Reports, 2023-04-17;42(4):112410.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Mast cells decrease efficacy of anti-angiogenic therapy by secreting matrix-degrading granzyme B
Authors: M Wroblewski, R Bauer, M Cubas Córd, F Udonta, I Ben-Batall, K Legler, C Hauser, J Egberts, M Janning, J Velthaus, C Schulze, K Pantel, C Bokemeyer, S Loges
Nat Commun, 2017-08-16;8(1):269.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Novel Recombinant DNA Vaccine Candidates for Human Respiratory Syncytial Virus: Preclinical Evaluation of Immunogenicity and Protection Efficiency
Authors: MA Farrag, HM Amer, P Öhlschläge, ME Hamad, FN Almajhdi
Hum Vaccin Immunother, 2017-03-08;0(0):0.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice.
Authors: Whitehead M, Ohlschlager P, Almajhdi F, Alloza L, Marzabal P, Meyers A, Hitzeroth I, Rybicki E
BMC Cancer, 2014-05-24;14(0):367.
Species: Mouse
Sample Types: Whole Cells
Applications: ELISA Development (Detection) -
Improved HSC reconstitution and protection from inflammatory stress and chemotherapy in mice lacking granzyme B.
Authors: Carnevalli L, Scognamiglio R, Cabezas-Wallscheid N, Rahmig S, Laurenti E, Masuda K, Jockel L, Kuck A, Sujer S, Polykratis A, Erlacher M, Pasparakis M, Essers M, Trumpp A
J Exp Med, 2014-04-21;211(5):769-79.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Hematopoietic stem cell function and survival depend on c-Myc and N-Myc activity.
Authors: Laurenti E, Varnum-Finney B, Wilson A, Ferrero I, Blanco-Bose WE, Ehninger A, Knoepfler PS, Cheng PF, MacDonald HR, Eisenman RN, Bernstein ID, Trumpp A
Cell Stem Cell, 2008-12-04;3(6):611-24.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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