Mouse HB-EGF Antibody Summary
Accession # Q06186
This antibody functions as an ELISA capture antibody when paired with Rat Anti-Mouse HB‑EGF Monoclonal Antibody (Catalog # MAB82391).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse HB-EGF DuoSet ELISA Kit (Catalog # DY8239-05) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Mouse HB‑EGF ELISA Standard Curve. Recombinant Mouse HB-EGF protein was serially diluted 2-fold and captured by Rat Anti-Mouse HB-EGF Monoclonal Antibody (Catalog # MAB8239) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse HB-EGF Monoclonal Antibody ( Catalog # MAB82391) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Heparin-binding EGF-like growth factor, HB-EGF, is a 19-23 kDa glycoprotein, member of the EGF family of mitogens that exists in multiple forms as a result of heterogenous O-glycosylation. HB-EGF is a growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. It is required for normal cardiac valve formation and normal heart function and promotes smooth muscle cell proliferation. HB-EGF may be involved in macrophage-mediated cellular proliferation. The cDNA for mouse Proheparin-binding EGF-like growth factor encodes a 208 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB‑EGF. Like EGF, TGF-alpha, and AR, HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF.
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