Mouse HB-EGF Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB82391-100
MAB82391-SP
Mouse HB‑EGF ELISA Standard Curve.
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Mouse HB-EGF Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse HB-EGF in direct ELISAs.
Source
Recombinant Monoclonal Rat IgG2A Clone # 891808R
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant mouse HB-EGF
Asp63-Leu148
Accession # Q06186
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse HB‑EGF Monoclonal Antibody (Catalog # MAB8239).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse HB-EGF DuoSet ELISA Kit (Catalog # DY8239-05) for convenient development of a sandwich ELISA.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Mouse HB‑EGF ELISA Standard Curve. Recombinant Mouse HB-EGF protein was serially diluted 2-fold and captured by Rat Anti-Mouse HB-EGF Monoclonal Antibody ( Catalog # MAB8239) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse HB-EGF Monoclonal Antibody (Catalog # MAB82391) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HB-EGF

Heparin-binding EGF-like growth factor, HB-EGF, is a 19-23 kDa glycoprotein, member of the EGF family of mitogens that exists in multiple forms as a result of heterogenous O-glycosylation.  HB-EGF is a growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. It is required for normal cardiac valve formation and normal heart function and promotes smooth muscle cell proliferation. HB-EGF may be involved in macrophage-mediated cellular proliferation. The cDNA for mouse Proheparin-binding EGF-like growth factor encodes a 208 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB‑EGF. Like EGF, TGF-alpha, and AR, HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF.

Long Name
Heparin Binding EGF-like Growth Factor
Entrez Gene IDs
1839 (Human); 15200 (Mouse)
Alternate Names
diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor); Dtr; DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor); Dts; DTSF; HBEGF; HB-EGF; Hegfl; heparin-binding EGF-like growth factor; heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse HB-EGF Antibody

There are currently no reviews for this product. Be the first to review Mouse HB-EGF Antibody and earn rewards!

Have you used Mouse HB-EGF Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review