Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HSF4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse HSF4
Gly343-Pro492
Accession # Q9R0L1

Specificity

Detects mouse HSF4 in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) HSF4 is observed and less than 5% cross-reactivity with recombinant mouse HSF1 and rhHSF2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse HSF4 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse HSF4

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSF4

HSF4 (heat shock factor 4) is a 55 kDa member of the HSF family of transcription factors. It is highly expressed in lens where it maintains lens integrity and blocks FGF synthesis. Mouse HSF4B is 492 amino acids in length. It contains one DNA binding region (aa 17‑122) and a Leu-zipper trimerization domain (hydrophobic repeat‑A/B) (aa 129‑203). Two additional potential isoforms exist. The first isoform, termed HSF4A, is transcriptionally inactive and shows a 45 aa substitution for aa 245‑319. The second isoform shows an 18 aa substitution for the first 78 amino acids of the N-terminus. Over aa 343‑492, mouse HSF4 shares 93% and 72% aa sequence identity with rat and human HSF4, respectively.

Long Name

Heat Shock Transcription Factor 4

Alternate Names

AT-HSFB1, ATHSF4, CTM, HSFB1

Entrez Gene IDs

3299 (Human); 26386 (Mouse)

Gene Symbol

HSF4

UniProt

Q9R0L1

Additional HSF4 Products

Product Documents for Mouse HSF4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse HSF4 Antibody

For research use only

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