Mouse IFN‑ beta Antibody

Name: Mouse IFN‑ beta Antibody
Brand: R&D Systems
SKU: 32401-1
Price: 529 USD
Availability: InStock

R&D Systems | Catalog # 32401-1

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Citations (4)

Product Documents

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Neutralization

Cited:

Neutralization, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all IFN-beta Primary Antibodies »

Product Specifications

Immunogen

Recombinant mouse IFN-beta

Specificity

Detects mouse IFN-beta.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Mouse IFN‑ beta Antibody

Application

Recommended Usage

Neutralization

One neutralization unit is the amount of antiserum required to neutralize one unit ofmouse interferon beta (Mu-IFN-beta ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) J. Virol. 37, 755-758] using L929 cells and EMCV as the challenge virus. In this antiviral assay for interferon, about 2.5 unit/ml of interferon is the quantity necessary to produce acytopathic effect of 50%. The units are determined by use of a laboratory standard calibrated with respect to the international reference standard for Mu-IFN-beta provided by the National Institutes of Health [Gb02-902-511]. This materialis prepared specifically for effective neutralization of Mu-IFN-beta.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.3 M Sodium Bicarbonate, 0.2 M Sodium Chloride.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).

Background: IFN-beta

IFN-beta (Interferon-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

References

Asselin-Paturel et al. (J Exp. Med, 2005) used 32400 in combination with Rabbit anti-mouse IFN-alpha (32100) to explore the role of Type I IFN in the migration and activation of dendritic cells. Kamath et al. (J. Immunol. 2005) used a similar cocktail to demonstrate that dendritic cell derived Type I IFN stimulates bystander T-cells.
Seimon et al. (PNAS, 2006) used 32400 to examine the role of IFN Beta in protecting LPS treated macrophages from apoptosis. Zheng et al. (J. Biol. Chem, 2006) used 32400 to examine the role of autocrine IFN Beta in RANKL stimulated iNOS expression in macrophages.