Mouse IFN-gamma APC-conjugated Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IFN‑ gamma in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) stimulated to induce Th1 cells or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma APC-conjugated Monoclonal Antibody (Catalog # IC485A) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on control antibody staining (Catalog # IC006A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of IFN‑ gamma in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either (A) stimulated for 4 hours with Cell Activation Cocktail 500x (5476) or (B) unstimulated were stained with Rat Anti-Mouse IFN-gamma APC-conjugated Monoclonal Antibody (Catalog # IC485A) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (FAB554P). Quadrant markers were set based on control antibody staining (IC006A). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Interferon-gamma (IFN-gamma, IFNG), also known as type II or Immune Interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine. Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa molecular weight variably glycosylated subunits. It shares 86% amino acid (aa) sequence identity with rat IFN-gamma, 38-44% with bovine, canine, cotton rat, equine, feline, human, porcine and rhesus IFN‑ gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction. IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key function in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis.
Citation for Mouse IFN-gamma APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis
Authors: EN Komegae, TA Souza, LZ Grund, C Lima, M Lopes-Ferr
PLoS ONE, 2017-02-24;12(2):e0171796.
Sample Types: Whole Cells
Applications: Flow Cytometry
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