Mouse IL-22 Antibody (140301R)

Detection of IL‑22 in Mouse Th17 Splenocytes by Flow Cytometry.

Mouse splenocytes (A) differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3e monoclonal antibody (MAB484, 10 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (100-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 6 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, or (B) resting, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). Quadrant markers were set based on isotype control antibody (MAB006). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules.