Mouse IL-28B/IFN-lambda 3 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects IL‑28B/IFN‑ lambda 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human IL-28A is observed. In Western blots, approximately 35% cross-reactivity (reducing conditions) and approximately 10% cross-reactivity (non-reducing conditions) with recombinant mouse IL-28A is observed. Neutralizes 100% of recombinant mouse IL-28A.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse IL‑28B/IFN‑ lambda 3
    Asp20-Val193
    Accession # Q8CGK6
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑28B/IFN-lambda 3 (Catalog # 1789-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑28B/IFN‑ lambda 3 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 400 ng/mL Recombinant Mouse IL‑28B/IFN‑ lambda 3.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑28B/IFN‑ lambda 3 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL‑28B/IFN‑ lambda 3 Antibody. Recombinant Mouse IL‑28B/IFN‑ lambda 3 (Catalog # 1789‑ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Mouse IL‑28B/
IFN‑ lambda 3 (400 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑28B/IFN‑ lambda 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1789). The ND50 is typically 2‑10 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-28B/IFN-lambda 3

IL-28A, IL-28B, and IL-29, also named interferon-lambda 2 (IFN-lambda 2), IFN-lambda 3, and IFN-lambda 1, respectively, are class II cytokine receptor ligands that are distantly related to members of the IL-10 family (11‑13% amino acid (aa) sequence identity) and the type I IFN family (15‑19% aa sequence identity) (1‑3). The genes encoding these three cytokines are localized to chromosome 19 and each is composed of multiple exons. The exon organization of these genes is also found in the IL-10 family genes but is distinct from the type I IFNs, which are encoded within a single exon. The expression of IL-28A, B, and IL-29 is induced by virus infection or double‑stranded RNA. All three cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up‑regulation of MHC class I antigen expression. The three proteins signal through the same heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta ) and a novel IL-28 receptor  alpha (IL-28 R alpha, also known as IFN-lambda R1). Ligand binding to the receptor complex induces Jak kinase activation and STAT1 and STAT2 tyrosine phosphorylation. The phosphorylated STAT1 and STAT2 complex with IFN‑regulatory factor 9 (IRF-9) to form the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex that is translocated to the nucleus. ISGF-3 binds to the IFN-stimulated response element (ISRE) present in the regulatory regions of the target genes. Mouse IL-28B cDNA encodes a 193 amino acid residue precursor protein with a putative 15 aa signal peptide. It shares 61%, 62%, and 52% aa sequence identity with human IL-28A, human IL-28B, and human IL-29, respectively.

  • References:
    1. Vilcek, J. (2003) Nature Immunol. 4:8.
    2. Sheppard, P. et al. (2003) Nature Immunol. 4:63.
    3. Kotenko, S.V. et al. (2003) Nature Immunol. 4:69.
     
  • Long Name:
    Interleukin 28B
  • Entrez Gene IDs:
    282617 (Human); 338374 (Mouse)
  • Alternate Names:
    interleukin-28B; IFN-lambda 3; IL28B; IL-28B; IL28C; interferon, lambda 3
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