Mouse IL-6 PE-conjugated Antibody

IC406P has been discontinued. View all IL-6 products.
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-6 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL‑6, recombinant porcine IL‑6, recombinant rat IL-6, rhIL-11, rhCT-1, or rhCLC is observed.
  • Source
    Monoclonal Rat IgG1 Clone # MP520F3
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    COS-7 African green monkey SV40 transformed kidney fibroblast-like cell line-derived recombinant mouse IL-6
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑6 in J774A.1 Mouse Cell Line by Flow Cytometry. LPS stimulated J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse IL‑6 PE‑conjugated Monoclonal Antibody (Catalog # IC406P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-6
IL-6 (Interleukin-6), also known as Interferon beta 2, is a secreted, 24-30 kDa member of the IL-6 family of proteins. In mouse, mature IL-6 is a highly glycosylated, monomeric polypeptide that is 187 amino acids (aa) in length, and shares 85% and 40% aa identity with rat and human IL-6, respectively. IL-6 expression is induced in a highly diverse group of cells including vascular and visceral smooth muscle cells, T helper cells, fibroblasts, astrocytes, endothelial cells, neurons, monocytes, microglia, pancreatic islet alpha -cells, osteoblasts, keratinocytes and white adipocytes. The receptor for IL-6 is composed of two distinct type I transmembrane glycoproteins, an 80-85 kDa ligand-binding IL-6R alpha subunit, and two signal-transducing 130-135 kDa gp130 subunits. Notably, the IL-6 system seems to exist as one of two states; there is a "classic signaling" state where IL-6 interacts with the transmembrane forms of both IL-6R alpha and gp130 in a signaling complex, and an IL-6 "trans-signaling" state, where IL-6 is bound to soluble IL-6R alpha in a circulating complex that subsequently interacts with transmembrane gp130. The "classic signaling" complex occurs on select cells such as hepatocytes, neutrophils and monocytes. The outcome of "classic signaling" is typically anti-inflammatory, promoting hepatocyte and pancreatic proliferation and regeneration, plus hepatic acute phase protein production. "Trans-signaling", by contrast, is very widespread, as a soluble IL-6R alpha :IL-6 complex can go almost everywhere and interact with the ubiquitously-expressed gp130. Relative to "classic signaling" however, this type of signaling is typically pro-inflammatory, and results in endothelial and smooth muscle cell activation, an inhibition of Treg differentiation, and a mononuclear cell infiltration into inflammatory environments.
  • Long Name:
    Interleukin 6
  • Entrez Gene IDs:
    3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
  • Alternate Names:
    B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
Related Research Areas

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