Detects mouse IL-6 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) IL‑6, recombinant porcine IL‑6, recombinant rat IL-6, rhIL-11, rhCT-1, or rhCLC is observed.
Monoclonal Rat IgG1 Clone # MP520F3
Protein A or G purified from hybridoma culture supernatant
Detection of IL‑6 in J774A.1 Mouse Cell Line by Flow Cytometry.
LPS stimulated J774A.1 mouse reticulum cell sarcoma macrophage cell line was stained with Rat Anti-Mouse IL‑6 PE‑conjugated Monoclonal Antibody (Catalog # IC406P, filled histogram) or isotype control antibody (Catalog # IC005P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
IL-6 (Interleukin-6), also known as Interferon beta 2, is a secreted,
24-30 kDa member of the IL-6 family of proteins. In mouse, mature IL-6 is a highly
glycosylated, monomeric polypeptide that is 187 amino acids (aa) in length, and
shares 85% and 40% aa identity with rat and human IL-6, respectively. IL-6 expression is induced in a highly diverse
group of cells including vascular and visceral smooth muscle cells, T helper
cells, fibroblasts, astrocytes, endothelial cells, neurons, monocytes,
microglia, pancreatic islet alpha -cells, osteoblasts, keratinocytes and white
adipocytes. The receptor for IL-6 is
composed of two distinct type I transmembrane glycoproteins, an 80-85 kDa
ligand-binding IL-6R alpha subunit, and two signal-transducing 130-135 kDa gp130
subunits. Notably, the IL-6 system seems
to exist as one of two states; there is a "classic signaling" state where IL-6
interacts with the transmembrane forms of both IL-6R alpha and gp130 in a signaling complex,
and an IL-6 "trans-signaling" state, where IL-6 is bound to soluble IL-6R alpha in a
circulating complex that subsequently interacts with transmembrane gp130. The "classic signaling" complex occurs on
select cells such as hepatocytes, neutrophils and monocytes. The outcome of "classic signaling" is typically
anti-inflammatory, promoting hepatocyte and pancreatic proliferation and
regeneration, plus hepatic acute phase protein production. "Trans-signaling",
by contrast, is very widespread, as a soluble IL-6R alpha :IL-6 complex can go almost
everywhere and interact with the ubiquitously-expressed gp130. Relative to "classic signaling" however, this
type of signaling is typically pro-inflammatory, and results in endothelial and
smooth muscle cell activation, an inhibition of Treg differentiation, and a
mononuclear cell infiltration into inflammatory environments.
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