Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Integrin beta 7 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 7
Glu20-His722
Accession # P26011

Specificity

Detects mouse Integrin beta 7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) Integrin  beta 1, rmIntegrin beta 2, recombinant human Integrin beta 3, and rmIntegrin beta 6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse Integrin  beta 7 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse lymph node single‑cell suspension

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Integrin  beta 7

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin beta 7

Integrin beta 7 is a 130 kDa type I transmembrane glycoprotein that belongs to the integrin beta chain family. It heterodimerizes with either Integrin alpha 4 or alpha E. Whereas, Integrin alpha E beta 7 is a receptor for E-Cadherin, Integrin alpha 4/ beta 7 is a receptor for fibronectin, MAdCAM 1 and VCAM1. The extracellular domain of mouse Integrin beta 7 shares 94% and 87% amino acid sequence identity with that of rat and human Integrin beta 7, respectively.

Alternate Names

ITGB7

Entrez Gene IDs

3695 (Human); 16421 (Mouse); 25713 (Rat); 102137082 (Cynomolgus Monkey)

Gene Symbol

ITGB7

UniProt

P26011

Additional Integrin beta 7 Products

Product Documents for Mouse Integrin  beta 7 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Integrin  beta 7 Antibody

For research use only

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