Mouse Lefty-2 Antibody

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Detection of Mouse Lefty-2 by Western Blot.
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Mouse Lefty-2 Antibody Summary

Species Reactivity
Detects mouse Lefty-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 65% cross-reactivity with recombinant mouse Lefty-1 is observed, and approximately 20% cross-reactivity with recombinant human Lefty-2 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
Chinese hamster ovary cell line CHO-derived recombinant mouse Lefty-2
Accession # P57785
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Lefty-2 antibody by Western Blot. View Larger

Detection of Mouse Lefty-2 by Western Blot. Western blot shows lysates of P19 mouse embryonal carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Mouse Lefty-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7648) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Lefty-2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Lefty-2 antibody in D3 Mouse Embryonic Stem Cells by Immunocytochemistry (ICC). View Larger

Lefty-2 in D3 Mouse Embryonic Stem Cells. Lefty-2 was detected in immersion fixed D3 mouse embryonic stem cells untreated (lower panel) or stimulated (upper panel) with recombinant human/mouse/rat Activin A (Catalog # 338-AC) using Sheep Anti-Mouse Lefty-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7648) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to the cytoplasm of Activin A-stimulated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lefty-2

Lefty-2 (Left-right determination factor 2; also Lefty-B [in human]) is a atypical member of the TGF-beta family of proteins. It is expressed during early embryogenesis in both the primitive streak and left-side lateral plate mesoderm. In the adult, Lefty-2 appears in oviduct epithelium. Lefty-2 acts in a manner reminiscent of that for Chordin and Noggin, and it is assumed that Lefty-2 is an antagonist of BMP activity. Notably, Lefty-2 and Nodal are likely under the control of Lefty-1, and thus all three molecules would appear to contribute to the creation of a left side-type body plan. Mouse Lefty-2 is synthesized as a 368 amino acid (aa) preproprecursor. It contains a 21 aa signal sequence, plus a 347 aa, 41-42 kDa bioactive proprecursor that may undergo proteolytic processing at one of two downstream cleavage sites. If cleavage occurs after Arg77, the resulting 33-34 kDa mature form (aa 78-368) is biologically inactive; if cleavage occurs after Arg135, the resulting 27-28 kDa mature form (aa 136-368) is biologically active. Lefty-2 is not a covalent homodimer and has been suggested to act as a monomer. Over aa 78-368, mouse Lefty-2 shares 94%, 83% and 95% aa sequence identity with rat Lefty-2, human Lefty-B and mouse Lefty-1, respectively.

Entrez Gene IDs
7044 (Human); 320202 (Mouse); 289316 (Rat)
Alternate Names
Ebaf; Endometrial Bleeding-Associated Factor; LEFTA; Left-Right Determination Factor 2; Left-right determination factor A; Lefty-2; LEFTYA; Protein Lefty-2; Protein Lefty-A; TGFB4; TGF-beta 4; TGF-beta-4; Transforming Growth Factor Beta-4

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