Mouse MGMT Antibody

Catalog # Availability Size / Price Qty
AF3299
AF3299-SP
Detection of Mouse MGMT by Western Blot.
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Mouse MGMT Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse MGMT in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse MGMT
Met1-Asn211
Accession # P26187
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse MGMT antibody by Western Blot. View Larger

Detection of Mouse MGMT by Western Blot. Western blot shows lysates of Balb/3T3 mouse embryonic fibroblast cell line and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse MGMT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3299) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MGMT at approximately 25 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MGMT

The O-6-methylguanine-DNA-methyl transferase (MGMT) is involved in the direct repair of DNA alkylated at the O6 position of guanine. MGMT transfers the alkyl group to its own cysteine thereby inactivating the MGMT molecule. Therefore, the number of MGMT molecules correlates directly with the cell’s ability to withstand DNA alkylation.

Long Name
O-6 Methylguanine-DNA Methyltransferase
Entrez Gene IDs
4255 (Human); 17314 (Mouse)
Alternate Names
6-O-methylguanine-DNA methyltransferase; EC 2.1.1.63; methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; O-6-methylguanine-DNA-alkyltransferase

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