Mouse Midkine Antibody

Catalog # Availability Size / Price Qty
AF7769
AF7769-SP
Detection of Mouse Midkine by Western Blot.
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Mouse Midkine Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Midkine in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human Midkine is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Midkine
Gly89-Asp140
Accession # P12025
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Midkine antibody by Western Blot. View Larger

Detection of Mouse Midkine by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7769) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Midkine at approximately 15-17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Midkine antibody in Mouse Embryo by Immunohistochemistry (IHC-P). View Larger

Midkine in Mouse Embryo. Midkine was detected in immersion fixed paraffin-embedded sections of mouse embryo (13 d.p.c.) using Sheep Anti-Mouse Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7769) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to cartilage primordium in ribs. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western Detection of Human and Mouse Midkine antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human and Mouse Midkine by Simple WesternTM. Simple Western lane view shows lysates of mouse embryo tissue, loaded at 0.2 mg/mL. A specific band was detected for Midkine at approximately 26 kDa (as indicated) using 20 µg/mL of Sheep Anti-Mouse Midkine Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7769) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Midkine

Midkine (MK; also Retinoid acid-induced differentiation facor) is a secreted heparin-binding member of the very small pleiotrophin family of proteins. Although its predicted MW is 13 kDa, it runs anomalously at 15-17 kDa in SDS-PAGE. MK is strongly expressed in the embryo, but is known to be secreted in the adult by endothelium, preadipocytes, proximal renal tubular epithelium, and CD4+ T cells. MK has multiple activities, including the inhibition of regulatory T cell production, the promotion of adipocyte formation, and the induction of chemokine production by smooth muscle, the clustering of Ach receptors on myoblasts, and the migration of embryonic neurons plus neutrophils and macrophages. It has multiple receptors, including heparin and chondroitin sulfate, LRP-1, nucleolin, ALK, and PTP-zeta. Mature mouse midkine is 118 amino acids (aa) in length (aa 23-140). It possesses two distinct domains, an N-terminal domain spanning aa 23-71, and a C-terminal domain that encompasses aa 81-140. The C-terminal domain is further divided into two basic amino acid clusters that bind heparin. There are two splice variants reported for mouse midkine. One shows a deletion of aa 39-103, while another shows a deletion of aa 80-133. Midkine will form a covalent, crosslinked homodimer through the action of tissue type II transglutaminase. Full-length mature mouse MK (aa 23-140) shares 97% and 86% aa sequence with full-length rat and human MK, respectively..

Entrez Gene IDs
4192 (Human); 17242 (Mouse)
Alternate Names
Amphiregulin-associated protein; ARAP; MDK; MEK; Midgestation and kidney protein; midkine (neurite growth-promoting factor 2); Midkine; MK1; MKARAP; NEGF2; NEGF2FLJ27379; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein

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