Mouse Neutrophil Elastase/ELA2 DuoSet ELISA

Catalog # Availability Size / Price Qty
DY4517-05
Ancillary Products Available
Mouse Neutrophil Elastase / ELA2 ELISA Standard Curve
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Product Details
Citations (10)
FAQs
Supplemental Products
Reviews (2)

Mouse Neutrophil Elastase/ELA2 DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Sample Volume Required
100 µL
Assay Range
12.5 - 800 pg/mL
Sufficient Materials
For five 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Mouse Neutrophil Elastase / ELA2 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.

Entrez Gene IDs:
1991 (Human); 50701 (Mouse)
Alternate Names:
Bone marrow serine protease; EC 3.4.21; EC 3.4.21.37; ELA2; ELA2granulocyte-derived elastase; ELANE; elastase 2, neutrophil; elastase, neutrophil expressed; Elastase-2; GE; HLEelastase-2; HNE; Human leukocyte elastase; Leukocyte Elastase; Medullasin; NE; Neutrophil Elastase; PMN elastase; PMN-E; polymorphonuclear elastase; SCN1

Citations for Mouse Neutrophil Elastase/ELA2 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Neutrophil Extracellular Traps Promote NLRP3 Inflammasome Activation and Glomerular Endothelial Dysfunction in Diabetic Kidney Disease
    Authors: A Gupta, K Singh, S Fatima, S Ambreen, S Zimmermann, R Younis, S Krishnan, R Rana, I Gadi, C Schwab, R Biemann, K Shahzad, V Rani, S Ali, PR Mertens, S Kohli, B Isermann
    Nutrients, 2022;14(14):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  2. CXCR4 and CXCR7 Inhibition Ameliorates the Formation of Platelet-Neutrophil Complexes and Neutrophil Extracellular Traps through Adora2b Signaling
    Authors: KC Ngamsri, RA Putri, C Jans, K Schindler, A Fuhr, Y Zhang, J Gamper-Tsi, S Ehnert, FM Konrad
    International Journal of Molecular Sciences, 2021;22(24):.
    Species: Mouse
    Sample Types: Peritoneal Lavage Fluid
  3. PAD4-dependent NETs generation are indispensable for intestinal clearance of Citrobacter rodentium
    Authors: P Saha, BS Yeoh, X Xiao, RM Golonka, V Singh, Y Wang, M Vijay-Kuma
    Mucosal Immunol, 2019;0(0):.
    Species: Mouse
    Sample Types: Serum
  4. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis
    Authors: G Wen, W An, J Chen, EM Maguire, Q Chen, F Yang, SWA Pearce, M Kyriakides, L Zhang, S Ye, S Nourshargh, Q Xiao
    J Am Heart Assoc, 2018;7(4):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  5. Myocardial Ischemia Reperfusion Injury: Apoptotic, Inflammatory and Oxidative Stress Role of Galectin-3
    Authors: S Al-Salam, S Hashmi
    Cell. Physiol. Biochem., 2018;50(3):1123-1139.
    Species: Mouse
    Sample Types: Tissue Homogenates
  6. Phospholipid transfer protein and alpha-1 antitrypsin regulate Hck kinase activity during neutrophil degranulation
    Authors: P Ochieng, S Nath, R Macarulay, E Eden, A Dabo, M Campos, XC Jiang, RF Foronjy, P Geraghty
    Sci Rep, 2018;8(1):15394.
    Species: Mouse
    Sample Types: Cell Lysates
  7. Myeloperoxidase deficiency attenuates systemic and dietary iron-induced adverse effects
    Authors: X Xiao, P Saha, BS Yeoh, JA Hipp, V Singh, M Vijay-Kuma
    J. Nutr. Biochem., 2018;62(0):28-34.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Heme scavenging reduces pulmonary endoplasmic reticulum stress, fibrosis, and emphysema
    Authors: S Aggarwal, I Ahmad, A Lam, MA Carlisle, C Li, JM Wells, SV Raju, M Athar, SM Rowe, MT Dransfield, S Matalon
    JCI Insight, 2018;3(21):.
    Species: Mouse
    Sample Types: BALF
  9. Recombinant human IL-26 facilitates the innate immune response to endotoxin in the bronchoalveolar space of mice in vivo
    Authors: A Bao, KF Che, S Bozinovski, J Ji, JA Gregory, S Kumlien Ge, M Adner, LO Cardell, A Lindén
    PLoS ONE, 2017;12(12):e0188909.
    Species: Mouse
    Sample Types: BALF
  10. Silencing salusin-? attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats
    Authors: XS Ren, L Ling, B Zhou, Y Han, YB Zhou, Q Chen, YH Li, YM Kang, GQ Zhu
    Sci Rep, 2017;7(0):43259.
    Species: Rat
    Sample Types: Plasma

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Reviews for Mouse Neutrophil Elastase/ELA2 DuoSet ELISA

Average Rating: 5 (Based on 2 Reviews)

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Mouse Neutrophil Elastase/ELA2 DuoSet ELISA
By Anonymous on 10/12/2021
Sample Tested: Citrate Plasma

Mouse Neutrophil Elastase/ELA2 DuoSet ELISA
By Anonymous on 10/12/2021
Sample Tested: Citrate Plasma

We diluted the plasma 1:25