Mouse Neutrophil Elastase/ELA2 DuoSet ELISA

R&D Systems | Catalog # DY4517-05

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

12.5-800 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Mouse

Mouse Neutrophil Elastase/ELA2 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all Neutrophil Elastase/ELA2 ELISA Kits »

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Mouse Neutrophil Elastase/ELA2 DuoSet ELISA

Mouse Neutrophil Elastase / ELA2 ELISA Standard Curve

Mouse Neutrophil Elastase / ELA2 ELISA Standard Curve

Kit Contents for Mouse Neutrophil Elastase/ELA2 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Neutrophil Elastase/ELA2

Neutrophil Elastase (ELA2), also known as polymorphonuclear leukocyte elastase, is a serine protease belonging to the chymotrypsin family. Located primarily in the azurophil granules of polymorphonuclear leukocytes, ELA2 function results in the degradation of many extracellular matrix macromolecules. Alpha-1 Antitrypsin (Serpin A1) and secretory leukocyte protease inhibitor (SLPI) have been shown to inhibit ELA2 activity. ELA2 may be involved in lung emphysema, cystic fibrosis, the adult respiratory distress syndrome (ARDS), rheumatoid arthritis, tumor invasion and infectious diseases.

Alternate Names

ELA2, ELANE, Elastase-2, Leukocyte Elastase, Medullasin

Entrez Gene IDs

1991 (Human); 50701 (Mouse)

Gene Symbol

ELANE

Additional Neutrophil Elastase/ELA2 Products

Product Documents for Mouse Neutrophil Elastase/ELA2 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Neutrophil Elastase/ELA2 DuoSet ELISA

For research use only

Citations for Mouse Neutrophil Elastase/ELA2 DuoSet ELISA

Customer Reviews for Mouse Neutrophil Elastase/ELA2 DuoSet ELISA (2)

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  • Mouse Neutrophil Elastase/ELA2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Citrate Plasma
    Verified Customer | Posted 10/12/2021
    Mouse Neutrophil Elastase/ELA2 DuoSet ELISA DY4517-05
  • Mouse Neutrophil Elastase/ELA2 DuoSet ELISA
    Name: Anonymous
    Sample Tested: Citrate Plasma
    Verified Customer | Posted 10/12/2021
    We diluted the plasma 1:25
    Mouse Neutrophil Elastase/ELA2 DuoSet ELISA DY4517-05

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