Mouse OCAM/NCAM2 Antibody

R&D Systems | Catalog # AF778

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunoprecipitation, Bioassay, Mass Spectrometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all OCAM/NCAM2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse OCAM
Leu20-Gly700
Accession # O35136

Specificity

Detects mouse OCAM in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) NCAM-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse OCAM/NCAM2 Antibody

Detection of OCAM/NCAM2 by Immunohistochemistry

Detection of OCAM/NCAM2 by Immunohistochemistry

Infectious findings in the lungs and nasal cavity. (A) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) gene detection with RT- quantitative polymerase chain reaction (*P < 0.05). (B) Immunohistochemistry staining of SARS-CoV-2 in the lung (shown in brown, arrow heads). (C, D) Representative images of the olfactory epithelium from control hamsters (C: hematoxylin and eosin stain, D: immunohistological staining of NQO1 and OCAM). The boxes in (C) indicate the regions of the olfactory epithelium shown in D; the dorsal nasal septum (DS) area, ventral nasal septum (VS) area, dorsal lateral turbinate (DLT) area, and lateral turbinate (LT) area. Only the DS area appears positive for NQO1. (E, F) Representative images of the olfactory epithelium from SARS-CoV-2 hamsters. (G) Immunohistochemistry staining of the olfactory epithelium in SARS-CoV-2 hamsters. The virus is mainly present in the lateral region of the nasal cavity. (H) Double stained images of SARS-CoV-2 and OMP in each region of the olfactory epithelium. No double positive cells for SARS-CoV-2 and OMP are present in the olfactory epithelium. The arrow heads show SARS-CoV-2 positive cells. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fcimb.2022.924725/full), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of OCAM/NCAM2 by Immunohistochemistry

Detection of OCAM/NCAM2 by Immunohistochemistry

Infectious findings in the lungs and nasal cavity. (A) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) gene detection with RT- quantitative polymerase chain reaction (*P < 0.05). (B) Immunohistochemistry staining of SARS-CoV-2 in the lung (shown in brown, arrow heads). (C, D) Representative images of the olfactory epithelium from control hamsters (C: hematoxylin and eosin stain, D: immunohistological staining of NQO1 and OCAM). The boxes in (C) indicate the regions of the olfactory epithelium shown in D; the dorsal nasal septum (DS) area, ventral nasal septum (VS) area, dorsal lateral turbinate (DLT) area, and lateral turbinate (LT) area. Only the DS area appears positive for NQO1. (E, F) Representative images of the olfactory epithelium from SARS-CoV-2 hamsters. (G) Immunohistochemistry staining of the olfactory epithelium in SARS-CoV-2 hamsters. The virus is mainly present in the lateral region of the nasal cavity. (H) Double stained images of SARS-CoV-2 and OMP in each region of the olfactory epithelium. No double positive cells for SARS-CoV-2 and OMP are present in the olfactory epithelium. The arrow heads show SARS-CoV-2 positive cells. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fcimb.2022.924725/full), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse OCAM/NCAM2 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse OCAM

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OCAM/NCAM2

OCAM is a homophilic adhesion molecule belonging to the immunoglobulin superfamily and is expressed by a subset of both olfactory and vomeronasal axons in a zone-specific manner. OCAM is also known as neural cell adhesion molecule 2 (NCAM-2) and Rb-8 neural cell adhesion molecule (RNCAM).

Long Name

Opioid-binding and Cell Adhesion Molecule/Neural Cell Adhesion Molecule 2

Alternate Names

NCAM2, Rb-8, RNCAM

Entrez Gene IDs

4685 (Human); 17968 (Mouse)

Gene Symbol

NCAM2

UniProt

O35136

Additional OCAM/NCAM2 Products

Product Documents for Mouse OCAM/NCAM2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse OCAM/NCAM2 Antibody

For research use only

Related Research Areas

Immunoglobulin Superfamily CAMs

Citations for Mouse OCAM/NCAM2 Antibody

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