Mouse OCILRP2/CLEC2i Antibody

Catalog # Availability Size / Price Qty
AF3370
AF3370-SP
Detection of Mouse OCILRP2/CLEC2i by Immunocytochemistry/Immunofluorescence
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Mouse OCILRP2/CLEC2i Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse OCILRP2/CLEC2i in direct ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse OCIL is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse OCILRP2/CLEC2i
Thr77-Val217
Accession # Q9WVF9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse OCILRP2/CLEC2i

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry/ Immunofluorescence Detection of Mouse OCILRP2/CLEC2i by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse OCILRP2/CLEC2i by Immunocytochemistry/Immunofluorescence Membrane co-localization of OCILRP2 and DAP12 under anti-CD3/CD28 mAb treatment in EL4 T cells.EL4 T cells pre-transfected with pEGFP-siOCILRP2 or pre-coated with the OCILRP2 Ab were cultured overnight in the presence or absence of an anti-CD3/CD28 mAb and then stained for OCILRP2 (green) and nuclear stained with DAPI (blue) and DAP12 (red) to study OCILRP2 and DAP12 protein expression and localization. Unstimulated EL4 T cells exhibited OCILRP2 protein expression in the cytoplasm (upper panels). In contrast, CD3/CD28-activated EL4 T cells showed intracellular and membrane OCILRP2. OCILRP2/DAP12 co-localization appears in yellow. Each picture is representative of the vast majority of the observed cells on the slides. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0113218), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse OCILRP2/CLEC2i by Western Blot View Larger

Detection of Mouse OCILRP2/CLEC2i by Western Blot Interaction of OCILRP2 and DAP12 in GST pull-down and co-immunoprecipitation assays.The GST pull-down assay was carried out using purified beads that contained GST, GST-DAP12, or GST-OCILRP2. Precipitated OCILRP2 or DAP12 was detected by western blotting using an anti-OCILRP2 or anti-DAP12 antibody, respectively (a, b). 293T cells were grown in 6-cm dishes and transfected with the pCDNA3-HA-OCILRP2 and pDsRed-C1-DAP12 plasmids, respectively. OCILRP2 and DAP12 were detected by western blotting using an anti-HA antibody or an anti-DAP12 antibody (c). Schematic diagram of OCILRP2 predicted by SMART software. The green column represents the transmembrane region (amino acids 57–80) (d). The GST pull-down assay was carried out using purified beads that contained GST, full-length GST-OCILRP2, GST-OCILRP2i (aa 1–57), or GST-OCILRP2e (aa 80–221). Precipitated DAP12 was detected by western blotting using an anti-DAP12 antibody (e). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0113218), licensed under a CC-BY license. Not internally tested by R&D Systems.

Western Blot Detection of Mouse OCILRP2/CLEC2i by Western Blot View Larger

Detection of Mouse OCILRP2/CLEC2i by Western Blot Interaction of OCILRP2 and DAP12 in GST pull-down and co-immunoprecipitation assays.The GST pull-down assay was carried out using purified beads that contained GST, GST-DAP12, or GST-OCILRP2. Precipitated OCILRP2 or DAP12 was detected by western blotting using an anti-OCILRP2 or anti-DAP12 antibody, respectively (a, b). 293T cells were grown in 6-cm dishes and transfected with the pCDNA3-HA-OCILRP2 and pDsRed-C1-DAP12 plasmids, respectively. OCILRP2 and DAP12 were detected by western blotting using an anti-HA antibody or an anti-DAP12 antibody (c). Schematic diagram of OCILRP2 predicted by SMART software. The green column represents the transmembrane region (amino acids 57–80) (d). The GST pull-down assay was carried out using purified beads that contained GST, full-length GST-OCILRP2, GST-OCILRP2i (aa 1–57), or GST-OCILRP2e (aa 80–221). Precipitated DAP12 was detected by western blotting using an anti-DAP12 antibody (e). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0113218), licensed under a CC-BY license. Not internally tested by R&D Systems.

Flow Cytometry Detection of Mouse OCILRP2/CLEC2i by Flow Cytometry View Larger

Detection of Mouse OCILRP2/CLEC2i by Flow Cytometry Membrane re-localization of OCILRP2 via treatment with an anti-CD3/CD28 mAb.EL4 cells were incubated with plate-bound CD3/CD28 antibodies, isotype-matched mIgGs, or PMA/ionomycin for 48 h. The cells (1×106 cells) were then incubated with an anti-OCILRP2 antagonist antibody for 30 min on ice, followed by staining with an FITC-labeled second antibody. The cells were analyzed with Cellquest software using a FACS Calibur flow cytometer. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0113218), licensed under a CC-BY license. Not internally tested by R&D Systems.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: OCILRP2/CLEC2i

OCILRP2, also known as CLRG (C-type lectin related g) and CLEC2i (C-type lectin domain family 2, member i), is a type II transmembrane protein whose gene has been mapped to the natural killer gene complex (NKC) on mouse chromosome 6. By alternative splicing, multiple isoforms exist. OCIL RP2 is expressed on osteoclasts, chondrocytes and lymphoid cell types. It is a ligand for NKrp1f (KLRB1f), another NK receptor on the NKC. OCILRP2 inhibits osteoclast formation and can affect NK cell functions. The extracellular domain of mouse OCILRP2 shares 79% and 74% amino acid sequence identity with that of mouse OCILRP1 and OCIL, respectively. A human OCILRP2 ortholog has not been identified.

Long Name
Osteoclast Inhibitory Lectin Related Protein 2
Entrez Gene IDs
93675 (Mouse)
Alternate Names
CLAX; CLEC2i; CLRG; LLT1; OCIL; OCILRP2

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