Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PCPE-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PCPE-1
Gln25-Ala468 (predicted)
Accession # Q61398

Specificity

Detects mouse PCPE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 35% cross-reactivity with recombinant human PCPE is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse PCPE‑1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse PCPE‑1, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse PCPE‑1

Reviewed Applications

Read 1 review rated 5 using AF2239 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PCPE-1

PCPE-1 is a secreted 55 kDa enzyme adaptor that enhances the activity of the BMP-1/tolloid family of metalloproteinases (also known as procollagen C-proteinases) on fibrillar procollagens. PCPE-1 binds to the C-propeptide region and also elsewhere in the procollagen molecule to specifically stimulate BMP-1/tolloid processing of procollagen I, II, and III.

Long Name

Procollagen C-Proteinase Enhancer-1

Alternate Names

PCOLCE, PCPE1

Entrez Gene IDs

5118 (Human); 18542 (Mouse)

Gene Symbol

PCOLCE

UniProt

Q61398

Additional PCPE-1 Products

Product Documents for Mouse PCPE‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse PCPE‑1 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Mouse PCPE‑1 Antibody

Customer Reviews for Mouse PCPE‑1 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
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4 Stars
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2 Stars
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1 Stars
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  • Human Marapsin/Pancreasin Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Recombinant proteins and Heart lysates
    Species: Rat
    Verified Customer | Posted 09/14/2021
    Data collected using AF2239 against two types of samples: one with expression of PCPE1 and one with very low level of PCPE1
    Use antibody at 10 fold dilution (mAb saturation signal); Antibody test on recombinant rat PCE1 and lysate of rat heart.
    Mouse PCPE‑1 Antibody AF2239
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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