Mouse PCPE-1 Antibody

R&D Systems | Catalog # AF2239

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PCPE-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PCPE-1
Gln25-Ala468 (predicted)
Accession # Q61398

Specificity

Detects mouse PCPE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 35% cross-reactivity with recombinant human PCPE is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse PCPE-1 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse PCPE‑1, see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse PCPE‑1

Reviewed Applications

Read 1 review rated 5 using AF2239 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PCPE-1

PCPE-1 is a secreted 55 kDa enzyme adaptor that enhances the activity of the BMP-1/tolloid family of metalloproteinases (also known as procollagen C-proteinases) on fibrillar procollagens. PCPE-1 binds to the C-propeptide region and also elsewhere in the procollagen molecule to specifically stimulate BMP-1/tolloid processing of procollagen I, II, and III.

Long Name

Procollagen C-Proteinase Enhancer-1

Alternate Names

PCOLCE, PCPE1

Entrez Gene IDs

5118 (Human); 18542 (Mouse)

Gene Symbol

PCOLCE

UniProt

Q61398

Additional PCPE-1 Products

Product Documents for Mouse PCPE-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse PCPE-1 Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Mouse PCPE-1 Antibody

Customer Reviews for Mouse PCPE-1 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
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4 Stars
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2 Stars
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1 Stars
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  • Human Marapsin/Pancreasin Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Recombinant proteins and Heart lysates
    Species: Rat
    Verified Customer | Posted 09/14/2021
    Data collected using AF2239 against two types of samples: one with expression of PCPE1 and one with very low level of PCPE1
    Use antibody at 10 fold dilution (mAb saturation signal); Antibody test on recombinant rat PCE1 and lysate of rat heart.
    Mouse PCPE-1 Antibody AF2239
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.

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