Detects mouse PDGF R beta in Western blots. In Western blots, approximately 40% cross‑reactivity with recombinant human PDGF R beta is observed and less than 1% cross-reactivity with recombinant mouse PDGF R alpha is observed.
Polyclonal Goat IgG
Mouse myeloma cell line NS0-derived recombinant mouse PDGF R beta Leu32-Lys530 Accession # P05622
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
PDGF R beta in Mouse Embryo.
PDGF R beta was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse PDGF R beta Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1042) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF R beta
The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero‑dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero‑dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1‑4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3-ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta receptors. Others have only the alpha receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets and megakaryocytes) or only the beta receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells and macrophages). A soluble PDGF R alpha has been detected in normal human plasma and serum as well as in the conditioned medium of the human osteosarcoma cell line MG-63 (5). Both the recombinant mouse and human soluble PDGF R alpha bind PDGF with high affinity and are potent PDGF antagonists.
Betshotz, C. et al. (2001) BioEssays 23:494.
Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
Tiesman, J. and C.E. Hart (1993) J. Biol. Chem. 5:9621.
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