Mouse PDGF R beta Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF1042

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PDGF R beta  in Mouse Embryo.
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Citations (7)
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Mouse PDGF R beta Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse PDGF R beta in Western blots. In Western blots, approximately 40% cross‑reactivity with recombinant human PDGF R beta  is observed and less than 1% cross-reactivity with recombinant mouse PDGF R alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse PDGF R beta
Leu32-Lys530
Accession # P05622
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse PDGF R beta Fc Chimera (Catalog # 1042-PR)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry PDGF R beta  antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

PDGF R beta in Mouse Embryo. PDGF R beta was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse PDGF R beta Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1042) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF R beta

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero‑dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero‑dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1‑4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3-ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta  receptors. Others have only the alpha  receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets and megakaryocytes) or only the beta  receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells and macrophages). A soluble PDGF R alpha has been detected in normal human plasma and serum as well as in the conditioned medium of the human osteosarcoma cell line MG-63 (5). Both the recombinant mouse and human soluble PDGF R alpha bind PDGF with high affinity and are potent PDGF antagonists.

References
  1. Betshotz, C. et al. (2001) BioEssays 23:494.
  2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
  3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
  4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
  5. Tiesman, J. and C.E. Hart (1993) J. Biol. Chem. 5:9621.
Long Name
Platelet-derived Growth Factor Receptor beta
Entrez Gene IDs
5159 (Human); 18596 (Mouse); 24629 (Rat)
Alternate Names
beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide

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Citations for Mouse PDGF R beta Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. ASPSCR1::TFE3 orchestrates the angiogenic program of alveolar soft part sarcoma
    Authors: M Tanaka, S Chuaychob, M Homme, Y Yamazaki, R Lyu, K Yamashita, K Ae, S Matsumoto, K Kumegawa, R Maruyama, W Qu, Y Miyagi, R Yokokawa, T Nakamura
    Nature Communications, 2023-04-07;14(1):1957.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Runx1 and Runx2 inhibit fibrotic conversion of cellular niches for hematopoietic stem cells
    Authors: Y Omatsu, S Aiba, T Maeta, K Higaki, K Aoki, H Watanabe, G Kondoh, R Nishimura, S Takeda, UI Chung, T Nagasawa
    Nature Communications, 2022-05-12;13(1):2654.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. MDS cells impair osteolineage differentiation of MSCs via extracellular vesicles to suppress normal hematopoiesis
    Authors: Y Hayashi, KC Kawabata, Y Tanaka, Y Uehara, Y Mabuchi, K Murakami, A Nishiyama, S Kiryu, Y Yoshioka, Y Ota, T Sugiyama, K Mikami, M Tamura, T Fukushima, S Asada, R Takeda, Y Kunisaki, T Fukuyama, K Yokoyama, T Uchida, M Hagihara, N Ohno, K Usuki, A Tojo, Y Katayama, S Goyama, F Arai, T Tamura, T Nagasawa, T Ochiya, D Inoue, T Kitamura
    Cell Reports, 2022-05-10;39(6):110805.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Cell Autonomous and Non-cell Autonomous Regulation of SMC Progenitors in Pulmonary Hypertension
    Authors: AQ Sheikh, FZ Saddouk, A Ntokou, R Mazurek, DM Greif
    Cell Rep, 2018-04-24;23(4):1152-1165.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Pericyte ALK5/TIMP3 Axis Contributes to Endothelial Morphogenesis in the Developing Brain
    Authors: JM Dave, T Mirabella, SD Weatherbee, DM Greif
    Dev. Cell, 2018-02-15;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  6. Prostacyclin prevents pericyte loss and demyelination induced by lysophosphatidylcholine in the central nervous system.
    Authors: Muramatsu R, Kuroda M, Matoba K, Lin H, Takahashi C, Koyama Y, Yamashita T
    J Biol Chem, 2015-03-20;290(18):11515-25.
    Species: Rat
    Sample Types: Whole Cells
    Applications: IHC-Fr
  7. Contribution of neural crest-derived cells in the embryonic and adult thymus.
    Authors: Foster K, Sheridan J, Veiga-Fernandes H, Roderick K, Pachnis V, Adams R, Blackburn C, Kioussis D, Coles M
    J. Immunol., 2008-03-01;180(5):3183-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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