hh Mouse PDGF R beta Antibody AF1042: R&D Systems

Mouse PDGF R beta Antibody

(15 citations)
(2 Reviews)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse PDGF R beta in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 35% cross-reactivity with recombinant human PDGF R beta.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse PDGF R beta
    Leu32-Lys530
    Accession # P05622
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.25 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Rat and Mouse PDGF R beta by Western Blot. Western blot shows lysates of rat spinal cord tissue, rat brain tissue, and mouse brain tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
PDGF R beta in Mouse Embryonic Spinal Cord. PDGF R beta was detected in immersion fixed frozen sections of mouse embryo spinal cord using 5 µg/mL Goat Anti-Mouse PDGF R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1042) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDGF R beta

The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and a heterodimer (PDGF-AB) (1, 2). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptors (PDGF R alpha and R beta ). Whereas alpha / alpha homo-dimerization is induced by PDGF-AA, -BB, -CC, and -AB, alpha / beta hetero-dimerization is induced by PDGF-AB, -BB, -CC, and -DD, and beta / beta homo-dimerization is induced only by PDGF-BB, and -DD (1 - 4). Both PDGF R alpha and R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3-ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. Ligand-induced receptor dimerization results in autophosphorylation in trans resulting in the activation of several intracellular signaling pathways that can lead to cell proliferation, cell survival, cytoskeletal rearrangement, and cell migration. Many cell types, including fibroblasts and smooth muscle cells, express both the alpha and beta  receptors. Others have only the alpha receptors (oligodendrocyte progenitor cells, mesothelial cells, liver sinusoidal endothelial cells, astrocytes, platelets and megakaryocytes) or only the beta  receptors (myoblasts, capillary endothelial cells, pericytes, T cells, myeloid hematopoietic cells and macrophages). A soluble PDGF R alpha has been detected in normal human plasma and serum as well as in the conditioned medium of the human osteosarcoma cell line MG-63 (5). Both the recombinant mouse and human soluble PDGF R alpha bind PDGF with high affinity and are potent PDGF antagonists.

  • References:
    1. Betshotz, C. et al. (2001) BioEssays 23:494.
    2. Ostman, A. and A.H. Heldin (2001) Advances in Cancer Research 80:1.
    3. Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
    4. LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
    5. Tiesman, J. and C.E. Hart (1993) J. Biol. Chem. 5:9621.
  • Long Name:
    Platelet-derived Growth Factor Receptor beta
  • Entrez Gene IDs:
    5159 (Human); 18596 (Mouse); 24629 (Rat)
  • Alternate Names:
    beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; CD140b antigen; CD140b; EC 2.7.10; EC 2.7.10.1; JTK12; PDGF R beta; PDGFR; PDGFR1; PDGFRB; PDGF-R-beta; platelet-derived growth factor receptor, beta polypeptide
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Assay to visualize specific protein oxidation reveals spatio-temporal regulation of SHP2
    Authors: R Tsutsumi, J Harizanova, R Stockert, K Schröder, PIH Bastiaens, BG Neel
    Nat Commun, 2017;8(1):466.
    Species: Mouse
    Sample Type: Whole Cells
    Application: WB
  2. Microglia protect against brain injury and their selective elimination dysregulates neuronal network activity after stroke
    Nat Commun, 2016;7(0):11499.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  3. Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
    Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R
    Dev Biol, 2016;409(1):218-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  4. Dual effects of carbon monoxide on pericytes and neurogenesis in traumatic brain injury
    Nat Med, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - PFA fixed
  5. Emergence of a Wave of Wnt Signaling that Regulates Lung Alveologenesis by Controlling Epithelial Self-Renewal and Differentiation
    Cell Rep, 2016;17(9):2312-2325.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Paraffin embedded
  6. VCAM1 acts in parallel with CD69 and is required for the initiation of oligodendrocyte myelination
    Nat Commun, 2016;7(0):13478.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Nogo receptor blockade overcomes remyelination failure after white matter stroke and stimulates functional recovery in aged mice
    Proc. Natl. Acad. Sci. U.S.A, 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  8. Decellularized zebrafish cardiac extracellular matrix induces mammalian heart regeneration
    Authors: WC Chen, Z Wang, MA Missinato, DW Park, DW Long, HJ Liu, X Zeng, NA Yates, K Kim, Y Wang
    Sci Adv, 2016;2(11):e1600844.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Frozen
  9. Enhanced sphingosine-1-phosphate receptor 2 expression underlies female CNS autoimmunity susceptibility.
    Authors: Cruz-Orengo L, Daniels B, Dorsey D, Basak S, Grajales-Reyes J, McCandless E, Piccio L, Schmidt R, Cross A, Crosby S, Klein R
    J Clin Invest, 2014;124(6):2571-84.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  10. Restoration of oligodendrocyte pools in a mouse model of chronic cerebral hypoperfusion.
    Authors: McQueen, Jamie, Reimer, Michell, Holland, Philip R, Manso, Yasmina, McLaughlin, Mark, Fowler, Jill H, Horsburgh, Karen
    PLoS ONE, 2014;9(2):e87227.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  11. Malignant stroma increases luminal breast cancer cell proliferation and angiogenesis through platelet-derived growth factor signaling.
    Authors: Pinto, Mauricio, Dye, Wendy W, Jacobsen, Britta M, Horwitz, Kathryn
    BMC Cancer, 2014;14(0):735.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  12. Pericyte loss influences Alzheimer-like neurodegeneration in mice.
    Authors: Sagare A, Bell R, Zhao Z, Ma Q, Winkler E, Ramanathan A, Zlokovic B
    Nat Commun, 2013;4(0):2932.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  13. Survival effect of PDGF-CC rescues neurons from apoptosis in both brain and retina by regulating GSK3beta phosphorylation.
    Authors: Tang Z, Arjunan P, Lee C, Li Y, Kumar A, Hou X, Wang B, Wardega P, Zhang F, Dong L, Zhang Y, Zhang SZ, Ding H, Fariss RN, Becker KG, Lennartsson J, Nagai N, Cao Y, Li X
    J. Exp. Med., 2010;207(4):867-80.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
  14. Characterization of neuroprogenitor cells expressing the PDGF beta-receptor within the subventricular zone of postnatal mice.
    Authors: Ishii Y, Matsumoto Y, Watanabe R, Elmi M, Fujimori T, Nissen J, Cao Y, Nabeshima Y, Sasahara M, Funa K
    Mol. Cell. Neurosci., 2007;37(3):507-18.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
    Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
    Clin. Cancer Res., 2006;12(9):2676-88.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Expand to show all 15 Citations
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Average Rating: 4.5 (Based on 2 reviews)

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We have 1 review tested in 1 species: Mouse.
We have 2 reviews tested in 2 applications: Immunocytochemistry/Immunofluorescence, Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunocytochemistry/Immunofluorescence Mouse PDGF R beta  Antibody AF1042
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Very Good
 ICC/IF Mouse Anonymous 11/02/2016
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Image Details
Immunocytochemistry/Immunofluorescence Mouse PDGF R beta  Antibody AF1042
Immunocytochemistry/Immunofluorescence: Mouse PDGF R beta Antibody [AF1042]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedBrain tissue
SpeciesMouse
  Excellent
 IHC Anonymous 12/16/2015
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Summary

ApplicationImmunohistochemistry
Sample TestedBrain tissue

Other Experimental Details

Other Experimental DetailsOptimisation protocols of Microwave and Pressure Cooker antigen retrievals at pH6 and pH9. Concentrations 1:100, 1:200 and 1:400. In FFPE rat brain tissue. Primary antibody incubation 1 hr.

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